Damaging effect of cumulus denudation on rabbit oocytes

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Presentation transcript:

Damaging effect of cumulus denudation on rabbit oocytes Jin-Hua Lu, M.Sc., Jun-Zuo Wang, M.Sc., Hui-Li Wang, M.Sc., Hong-Shu Sui, Ph.D., Yong Liu, M.Sc., Jing-Jing Li, M.Sc., Bo Liang, M.Sc., Jing-He Tan, Ph.D. Fertility and Sterility Volume 93, Issue 5, Pages 1567-1573 (March 2010) DOI: 10.1016/j.fertnstert.2009.05.066 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Microscopic photographs of rabbit oocytes after different treatments. (A–C) Images of lucifer yellow dye diffusion showing the open, partially open, and closed gap junctions between cumulus cells and the oocytes, respectively. (D–F) Images of Bcl-2 labeling showing intensive, mild, and slight Bcl-2 labeling, respectively. (G, H) Images of Hoechst 33258 staining of DNA showing oocytes that are unstained and stained, respectively. (I, J) Images of TUNEL staining showing TUNEL-positive DNA (arrows) in oocytes processed at 6 hours (the metaphase I stage) and 18 hours (corresponding to the metaphase II stage) of maturation culture, respectively. (K, K′) The same metaphase II oocyte with a first polar body (Big arrow) photographed under phase contrast and fluorescence, respectively, to show the TUNEL-negative (small arrow) but Hoechst 33342–positive DNA (Small arrow). Scale bar is 20 μm. Fertility and Sterility 2010 93, 1567-1573DOI: (10.1016/j.fertnstert.2009.05.066) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Electron photomicrographs of freshly collected mouse and rabbit oocytes. Although mouse oocytes have capacious perivitelline spaces (A) and few cell junctions always on the egg surface (arrows in C and E), rabbit oocytes show no perivitelline space (B) but many cell junctions that insert deeply into the egg cortex (arrows in B, D, and F). PVS = perivitelline space; ZP = zona pellucida; Oo = ooplasm. (Original magnifications: A, ×10,000; B, ×10,000; C and E, ×25,000; D and F, ×25,000.) Fertility and Sterility 2010 93, 1567-1573DOI: (10.1016/j.fertnstert.2009.05.066) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions