Lower levels of hsa-mir-15a, which decreases VEGFA, in the CD4+ T cells of pediatric patients with asthma Taiji Nakano, MD, Yuzaburo Inoue, MD, PhD,

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Lower levels of hsa-mir-15a, which decreases VEGFA, in the CD4+ T cells of pediatric patients with asthma Taiji Nakano, MD, Yuzaburo Inoue, MD, PhD, Naoki Shimojo, MD, PhD, Fumiya Yamaide, MD, PhD, Yoshinori Morita, MD, PhD, Takayasu Arima, MD, PhD, Minako Tomiita, MD, PhD, Yoichi Kohno, MD, PhD Journal of Allergy and Clinical Immunology Volume 132, Issue 5, Pages 1224-1227.e12 (November 2013) DOI: 10.1016/j.jaci.2013.06.041 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 CD4+ T cells of pediatric patients with asthma contained higher levels of VEGFA and lower levels of hsa-mir-15a, hsa-mir-15b, and hsa-mir-20a. A, VEGFA mRNA expression in peripheral blood CD4+ T cells. B, The correlation between hsa-mir-15a miR expression and VEGFA mRNA expression in the peripheral blood CD4+ T cells of the atopic asthma group. C, miR expression in peripheral blood CD4+ T cells. The lines and error bars are the medians and 25% to 75% percentiles, respectively (#P < .05, ##P < .01). Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Hsa-mir-15a decreased VEGFA mRNA expression levels. A and B, A part of the VEGFA 3′UTR containing either the putative binding site of hsa-mir-15a (intact VEGFA 3′UTR) or a mutated binding site (mutant VEGFA 3′UTR) was placed under the firefly luciferase gene in the pmirGLO vector. Cotransfection with hsa-mir-15a significantly decreased the normalized luciferase activity in intact VEGFA 3′UTR-transfected HEK293 cells (Fig 2, A, #P < .05, compared with control vector, n = 3) but not in the mutant VEGFA 3′UTR-transfected cells (Fig 2, B). C, Transfection of mature hsa-mir-15a but not mature control miR decreased endogenous VEGFA mRNA expression levels in HEK293 cells. The data are shown as the means ± SEM (##P < .01, compared with mature control miR, n = 4). Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Serum VEGF-A levels in pediatric patients with asthma and healthy control children. A, Serum VEGF-A levels were significantly higher in pediatric patients with asthma than in healthy control children. B, There was no difference in serum VEGF-A levels between pediatric patients with asthma using ICSs (ICS+) and those not using ICSs (ICS−). Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Expression of miRs that were predicted to bind VEGFA 3′UTR but were identically expressed in the atopic asthma group and the control groups. The expression levels of hsa-mir-16, -17, -20b, -29a, -29b, -29c, -106b, -195, and -424 were identical among the atopic asthma, atopic control, and nonatopic control groups. Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Correlation between hsa-mir-15b or -20a miR expression and VEGFA mRNA expression in the peripheral blood CD4+ T cells of the atopic asthma group. The expression levels of hsa-mir-15b and -20a were not significantly correlated with VEGFA mRNA expression in the peripheral blood CD4+ T cells of the atopic asthma group. Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Biotinylated hsa-mir-15a pull-down of VEGFA mRNA. HEK293 cells were transfected in quadruplicate with Bi-hsa-miR-15a (black) or Bi-cel-miR-67 (white). Enrichment of VEGFA mRNA or nontarget mRNA (ACTA2 and ORMDL3) was assessed by using quantitative RT-PCR relative to GAPDH mRNA. Values for the transcripts pulled down by Bi-hsa-miR-15a are shown as ratios to those pulled down by Bi-cel-miR-67. #P < .05. Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E5 Expression of VEGFA and hsa-mir-15a in peripheral blood CD4+ T cells was not associated with the usage of ICSs. There was no difference in the expression of VEGFA (A) and hsa-mir-15a (B) in peripheral blood CD4+ T cells between pediatric patients with asthma using ICSs (ICS+) and those not using ICSs (ICS−). Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E6 Correlation between hsa-mir-15a miR expression and total serum IgE in the atopic asthma group. Expression of hsa-mir-15a was not significantly correlated with total serum IgE in the atopic asthma group. Journal of Allergy and Clinical Immunology 2013 132, 1224-1227.e12DOI: (10.1016/j.jaci.2013.06.041) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions