Chk1 and 14‐3‐3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest Scatter plot shows the relative enrichment scores of common E2F7.

Slides:

Advertisements

Similar presentations

Individualized pain management plan.

Advertisements

Isotope‐based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation Reach‐scale conceptual model of the fluvial carbon.

Isotope‐based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation Sensitivity analysis of the ISOFLOC model displays.

Evening evapotranspirative cooling in relation to vegetation and urban geometry in the city of Ouagadougou, Burkina Faso Relation between sky view factor.

Matrilin‐2 prevents the TNFα‐induced apoptosis of WB‐F344 cells via suppressing JNK pathway Matrilin‐2 knockdown promotes the apoptosis of WB‐F344 cells.

Regulation of cargo‐selective endocytosis by dynamin 2 GTPase‐activating protein girdin AThe endocytic sites for the indicated cargoes were investigated.

KLF4 is a key determinant in the development and progression of cerebral cavernous malformations qRT–PCR analysis of Klf4 during disease progression at.

Defective immune responses in mice lacking LUBAC‐mediated linear ubiquitination in B cells Cpdm mice show residual LUBAC‐mediated linear polyubiquitination.

Details of the binding sites of tetracycline and edeine

Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α Protein immunoblotting of cell extracts and ConA precipitates.

The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication Representative images of wt and mdi1 ovarioles stained.

Co‐immunoprecipitation of Larp with MDI‐myc in transfected S2 cells

Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor‐mediated differentiation Components of the mitogen‐activated protein kinase (MAPK)

Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries Global Maps Presenting the Most.

CDC42 modulates the interaction between IRSp53 and VASP

Binding of p38α and p38β2 to MAP kinase kinases

The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes The stable MDA‐MB‐231 cell line expressing FLAG‐HaloTag‐WASH (FHT‐WASH)

The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes MDA‐MB‐231 cells expressing HaloTag WASH or CCDC53 were stained with.

Representative electron density maps

Retinoids activate proton transport by the uncoupling proteins UCP1 and UCP2 (A) pH dependency of the state 4 respiratory rate: control ▵ and UCP2 mitochondria.

Anthropogenic noise weakens territorial response to intruder's songs

Chk1 and 14‐3‐3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest Experimental procedures of the SILAC assay to identify binding.

The mutant p53‐ID4 complex controls VEGFA isoforms by recruiting lncRNA MALAT1 AWestern blot analysis of MDA‐MB‐468 cells interfered or not for hnRNP A1.

Prevalence of women with advanced maternal age by country.

Unanchored tri‐NEDD8 inhibits PARP‐1 to protect from oxidative stress‐induced cell death WT and NEDP1 KO HEK 293 cells were transfected with FLAG‐NSUN2.

Unanchored tri‐NEDD8 inhibits PARP‐1 to protect from oxidative stress‐induced cell death Non‐cullin neddylated species accumulate after oxidative stress.

Tousled‐like kinase 2 regulates recovery from a DNA damage‐induced G2 arrest U2OS cells were transfected with four independent siRNAs from the pools used.

Synergistic combination of valproic acid and oncolytic parvovirus H‐1PV as a potential therapy against cervical and pancreatic carcinomas VPA treatment.

Sequential treatment with 5‐aza‐2′‐deoxycytidine and deacetylase inhibitors reactivates HIV‐1 One night after cell purification, CD8+‐depleted PBMCs from.

Induced chromosome deletions cause hypersociability and other features of Williams–Beuren syndrome in mice Expression of genes measured by real‐time qRT‐PCR.

Regulation of a transcription factor network by Cdk1 coordinates late cell cycle gene expression A, B Cells expressing differentially tagged WT TFs (TOS4‐3FLAG.

Structural and functional analysis of the GABARAP interaction motif (GIM) Interaction profile of 30 biotinylated LIR peptides from various proteins against.

APhagocytosis assay of apoptotic or necrotic GFP‐expressing RAW264

DAP‐kinase is a Ca2+/calmodulin‐dependent, cytoskeletal‐associated protein kinase, with cell death‐inducing functions that depend on its catalytic activity.

Mammary stem cells and progenitors: targeting the roots of breast cancer for prevention (A) Schematic of the human breast highlighting terminal ductal.

Chk1 and 14‐3‐3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest ASchematic overview of the experimental setting and immunoblot.

Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae

A stele‐enriched gene regulatory network in the Arabidopsis root

Structural and functional analysis of the GABARAP interaction motif (GIM) GFP‐mCherry‐p62/SQSTM1 WT, T339V, L341V and T339V/L341V were overexpressed in.

Involvement of Crm1 in the hemin‐induced nuclear exclusion of Bach1

The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes MDA‐MB‐231 clones stably transfected with plasmids expressing control.

AMITOL added polyubiquitin chains to K481 of IRE1α

Heterogeneity in Δψmit reported by TMRE

Mitochondria are morphologically and functionally heterogeneous within cells Surface‐rendered three‐dimensional reconstructions of cells expressing mito‐DsRed1.

Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis Immunostaining of HeLa cells probed for FITC‐labeled streptavidin.

Confocal images representing the localization of PCDH7

Expression of Hcm1‐3N‐3HA and Hcm1‐8C‐3HA over the cell cycle

Cideb co‐precipitates with SCAP

Unanchored tri‐NEDD8 inhibits PARP‐1 to protect from oxidative stress‐induced cell death Western blot analysis of whole‐cell lysates from U2OS WT and NEDP1.

PARP‐1 activity is reduced in NEDP1 KO cells

The p97/VCP ATPase is critical in muscle atrophy and the accelerated degradation of muscle proteins The content of p97 and two of its cofactors are induced.

The p97/VCP ATPase is critical in muscle atrophy and the accelerated degradation of muscle proteins Electroporation of DNp97 in TA blocks both denervation.

Schematic diagram of MAPKKK5 constructs

A kinase‐dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion A, BGST‐Wapl‐N (A), or transiently expressed Myc‐Wapl.

Modulation of KSR activity in Caenorhabditis elegans by Zn ions, PAR‐1 kinase and PP2A phosphatase Elevated Zn2+ levels drastically increase the phosphorylation.

Repeat‐associated non‐AUG translation in C9orf72‐ALS/FTD is driven by neuronal excitation and stress AFilter‐trap binding assays show that the ISR proteins.

Zika virus inhibits type‐I interferon production and downstream signaling A549 cells were treated with IFN‐α (100 U/ml), IFN‐λ (200 ng/ml), or IFN–γ (10.

STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells IL‐3‐induced apoptosis in Ba/F3 cells expressing.

Processive movement of single kinesins on crowded microtubules visualized using quantum dots Effect of the mutant E164A on the motility of single quantum.

CNOT6L couples the selective degradation of maternal transcripts to meiotic cell cycle progression in mouse oocyte Relative mRNA copy number dynamics in.

The Escherichia coli RNA polymerase α subunit linker: length requirements for transcription activation at CRP‐dependent promoters Comparison of the effect.

Tumor suppressor Tsc1 is a new Hsp90 co‐chaperone that facilitates folding of kinase and non‐kinase clients Tsc1 was immunoprecipitated from HEK293 cell.

EglN2 associates with the NRF1‐PGC1α complex and controls mitochondrial function in breast cancer A, BqRT–PCR of mRNA from T47D transfected with either.

Imaging Disaster: Puerto Rico through the Eye of Hurricane María

CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression Immunoprecipitation of CD147 from RBL.

CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression The transmembrane and cytoplasmic regions.

Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy ATG16L1−/− cells reconstituted with ATG16L1WT or ATG16L1LE.

Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy Schematic presentation of ATG16L1 fragments and mutants.

RP58 controls neuron and astrocyte differentiation by downregulating the expression of Id1–4 genes in the developing cortex RP58 directly bound to the.

Notch inhibits apoptosis by direct interference with XIAP ubiquitination and degradation Interaction between XIAP and the endogenously generated Notch.

Presentation transcript:

Chk1 and 14‐3‐3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest Scatter plot shows the relative enrichment scores of common E2F7 and E2F8 binding partners. Red dashed line indicates the fold change cutoff (> 2.0). 14‐3‐3 isoforms are highlighted as red dots. 14‐3‐3 ζ interacts with wild‐type E2F7/8 but not with alanine mutants in vitro. HEK 293T cells were transfected with indicated constructs, and lysates were precipitated with GFP‐Trap beads and immunoblotted with antibodies directed against Myc or GFP. Inputs represent loading controls. Chromatin immunoprecipitation (ChIP) demonstrated that 14‐3‐3 proteins bind to the E2F7/8 target gene promoters. HEK cells were transfected with either PEI reagent alone (control) or indicated plasmids. 48 h after transfection, cells were harvested for ChIP assay and followed by qPCR. Histogram represents the enrichment ratio (bound/input) in E2Fs target gene promoters. A primer set designed against a distal region in the E2F1 gene served as a negative control. Luciferase reporter assay in U2OS cells using E2F1 promoter plasmid. Plasmids were co‐transfected either with wild‐type or alanine mutant, either alone or with 14‐3‐3 ζ plasmid. 14‐3‐3 inhibitor BV‐02 disrupts the interaction between 14‐3‐3 ζ and E2F7/8. Transfection was carried out as shown, and lysates were precipitated with GFP‐Trap beads and immunoblotted with antibodies directed against GFP or Myc. Inputs represent loading controls. Transcript levels of common E2F7 and E2F8 target genes after treatment with HU or HU + BV‐02 for 16 h, determined by qPCR. Data information: In (C, D and F), data represent average ± SEM (n = 3); *P < 0.05 (Student's t‐test) or n.s. (not significant).Source data are available online for this figure. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 37, Issue: 5, First published: 23 January 2018, DOI: (10.15252/embj.201797877)