Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulosa cells? Masako Karita, M.D., Yoshiki.

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Presentation transcript:

Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulosa cells? Masako Karita, M.D., Yoshiki Yamashita, M.D., Atsushi Hayashi, M.D., Yoko Yoshida, M.D., Mika Hayashi, M.D., Hikaru Yamamoto, M.D., Akiko Tanabe, M.D., Yoshito Terai, M.D., Masahide Ohmichi, M.D. Fertility and Sterility Volume 95, Issue 3, Pages 889-894 (March 2011) DOI: 10.1016/j.fertnstert.2010.12.026 Copyright © 2011 Terms and Conditions

Figure 1 (A) Comparison of mRNA expression of progesterone receptors (PRs) A and B. Expression of PR-A in patients with endometriosis was statistically higher than that in patients with tubal infertility (P<.01); however, there was no statistical difference in PR-B gene expression (P=.87). The relative expression ratio of each gene in granulosa cells was calculated by comparison with GAPDH expression. (B) Comparison of mRNA expression of estrogen receptors (ERs) α and β. ER-α mRNA expression in the endometriosis group was higher than that in the tubal infertility group (P=.04); however, ER-β showed no statistical difference (P=.86). The relative expression ratio of each gene in granulosa cells was calculated by comparison with GAPDH expression. (C) Comparison of mRNA expression of PR-A and ER-α between the pregnant and nonpregnant group. PR-A mRNA expression showed no statistical difference (P=.1). However, ER-α was statistically higher in the pregnancy group compared with the nonpregnancy group (P=.01). The relative expression ratio of each gene in granulosa cells was calculated by comparison with GAPDH expression. Fertility and Sterility 2011 95, 889-894DOI: (10.1016/j.fertnstert.2010.12.026) Copyright © 2011 Terms and Conditions

Figure 2 (Top) Correlation of mRNA expression of estrogen receptors (ERs) and progesterone receptor (PR) A in tubal infertility. In the tubal infertility group, a positive correlation between PR-A and ER mRNA expression was identified (R = 0.515 and 0.700, respectively). (Bottom) Correlation of mRNA expression of ERs and PR-B in tubal infertility. In the tubal infertility group, a positive correlation between PR-B and ER mRNA expression was identified (R = 0.545 and 0.602, respectively). The relative expression ratio of each gene in granulosa cells was calculated by comparison with GAPDH expression. Fertility and Sterility 2011 95, 889-894DOI: (10.1016/j.fertnstert.2010.12.026) Copyright © 2011 Terms and Conditions