Thymic stromal lymphopoietin does not activate human basophils

Slides:

Advertisements

Similar presentations

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Advertisements

Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2 Natalija Novak, MD, Nihal Mete, MD, Caroline.

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu Amanda J. Reefer, MS, Kathryn.

Human circulating group 2 innate lymphoid cells can express CD154 and promote IgE production Laura Maggi, PhD, Gianni Montaini, BSc, Alessio Mazzoni,

Mechanical injury polarizes skin dendritic cells to elicit a TH2 response by inducing cutaneous thymic stromal lymphopoietin expression Michiko K. Oyoshi,

Delayed clinical and ex vivo response to mammalian meat in patients with IgE to galactose-alpha-1,3-galactose Scott P. Commins, MD, PhD, Hayley R. James,

Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M

Akos Heinemann, MD, Gunter J. Sturm, MD, Martina Ofner, BSc, Eva M

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis Eva Gros, MSc, Susanne Petzold,

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Production of chemokines and proinflammatory and antiinflammatory cytokines by human alveolar macrophages activated by IgE receptors Philippe Gosset,

Histamine H2 receptor stimulation upregulates TH2 chemokine CCL17 production in human M2a macrophages Susanne Mommert, PhD, Karl Gregor, MD, Kristine.

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis Kaori Mukai, PhD, Nicolas Gaudenzio,

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Jennifer L. Ingram, PhD, Monica Kraft, MD

Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA Hirokazu Kinoshita, MD,

Is 9 more than 2 also in allergic airway inflammation?

Efficient cytokine-induced IL-13 production by mast cells requires both IL-33 and IL-3 Ilkka S. Junttila, MD, PhD, Cynthia Watson, BSc, Laura Kummola,

Responsiveness to respiratory syncytial virus in neonates is mediated through thymic stromal lymphopoietin and OX40 ligand Junyan Han, PhD, Azzeddine.

Selective and direct activation of human neutrophils but not eosinophils by Toll-like receptor 8 Markus Janke, PhD, Jens Poth, Vera Wimmenauer, Thomas.

Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska,

Mass cytometry profiling the response of basophils and the complete peripheral blood compartment to peanut Leticia Tordesillas, PhD, Adeeb H. Rahman,

Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines Maryam Salimi,

Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α John T. Schroeder, PhD,

Toll-like receptor 3 enhances late-phase reaction of experimental allergic conjunctivitis Mayumi Ueta, MD, PhD, Satoshi Uematsu, MD, PhD, Shizuo Akira,

Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps Sarah Peterson, MD, Julie A. Poposki, MS, Deepti.

Jovana Maric, MSc, Avinash Ravindran, MSc, Luca Mazzurana, MSc, Åsa K

Direct monitoring of basophil degranulation by using avidin-based probes Régis Joulia, PhD, Claire Mailhol, MD, Salvatore Valitutti, MD, Alain Didier,

Thymic stromal lymphopoietin and IL-33 modulate migration of hematopoietic progenitor cells in patients with allergic asthma Steven G. Smith, PhD, Akash.

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent Brittany M. Salter, BSc, John Paul Oliveria,

Dexamethasone regulation of thymic stromal lymphopoietin receptor expression on mast cells and their precursors Zoulfia Allakhverdi, PhD, Michael R.

Steroid resistance of airway type 2 innate lymphoid cells from patients with severe asthma: The role of thymic stromal lymphopoietin Sucai Liu, PhD,

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen- presenting cells that induce proallergic T cells Susanne Ebner, PhD,

Corticosteroid-resistant asthma is associated with classical antimicrobial activation of airway macrophages Elena Goleva, PhD, Pia J. Hauk, MD, Clifton.

T-box 21 transcription factor is responsible for distorted TH2 differentiation in human peripheral CD4+ T cells Osamu Kaminuma, DVM, PhD, Fujiko Kitamura,

Toll-like receptor 9, transmembrane activator and calcium-modulating cyclophilin ligand interactor, and CD40 synergize in causing B-cell activation Esra.

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Targeting allergen to FcγRI reveals a novel TH2 regulatory pathway linked to thymic stromal lymphopoietin receptor Kathryn E. Hulse, PhD, Amanda J. Reefer,

Fibronectin is a TH1-specific molecule in human subjects

A thymic stromal lymphopoietin–responsive dendritic cell subset mediates allergic responses in the upper airway mucosa Guro R. Melum, MD, Lorant Farkas,

Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A

Impaired natural killer cell functions in patients with signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations Giovanna.

A peptide derived from the Wiskott-Aldrich syndrome (WAS) protein-interacting protein (WIP) restores WAS protein level and actin cytoskeleton reorganization.

Zoulfia Allakhverdi, PhD, Michael R. Comeau, BSc, Dirk E

Thymic stromal lymphopoietin–activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis Wen Hao Wu, PhD,

Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis Deepti R. Nagarkar, PhD, Julie A. Poposki,

Fibronectin is a TH1-specific molecule in human subjects

Mammalian target of rapamycin inhibition counterbalances the inflammatory status of immune cells in patients with chronic granulomatous disease Aurélie.

Yilin Qi, MSc, Darwin J. Operario, PhD, Christopher M

Myeloid and plasmacytoid dendritic cells in induced sputum after allergen inhalation in subjects with asthma Benny Dua, MSc, Richard M. Watson, BSc,

Individual IL-3 priming is crucial for consistent in vitro activation of donor basophils in patients with chronic urticaria Thomas Gentinetta, MSc, Tatjana.

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

Peanut T-cell epitope discovery: Ara h 1

In vivo maturation of TH cells in relation to atopy

Staphylococcus aureus membrane and diacylated lipopeptide induce thymic stromal lymphopoietin in keratinocytes through the Toll-like receptor 2–Toll-like.

Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent Sharen Provoost, MSc, Tania.

Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity Iris Bellinghausen, PhD, Sebastian.

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Rhinovirus infection interferes with induction of tolerance to aeroantigens through OX40 ligand, thymic stromal lymphopoietin, and IL-33 Amit K. Mehta,

Short ragweed pollen triggers allergic inflammation through Toll-like receptor 4– dependent thymic stromal lymphopoietin/OX40 ligand/OX40 signaling pathways

Expression of chemokines and chemokine receptors in lesional and nonlesional upper skin of patients with atopic dermatitis Eva Gros, MSc, Caroline Bussmann,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Clinical safety of Food Allergy Herbal Formula-2 (FAHF-2) and inhibitory effect on basophils from patients with food allergy: Extended phase I study

IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients Antje Prasse, MD, Martin Germann,

Human basophils may not undergo modulation by DC-SIGN and mannose receptor– targeting immunotherapies due to absence of receptors Mrinmoy Das, MSc, Caroline.

Julie Negri, BA, S. Brandon Early, BA, John W

Functional assessment of pathogenic IgG subclasses in chronic autoimmune urticaria Sridharan Soundararajan, PhD, Yoko Kikuchi, DVM, Kusumam Joseph, PhD,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Presentation transcript:

Thymic stromal lymphopoietin does not activate human basophils Nina Salabert-Le Guen, PhD, Caroline Hémont, PharmD, PhD, Agathe Delbove, MD, Caroline Poli, MD, PhD, Cécile Braudeau, PhD, Aurélie Fantou, PharmD, Karine Amouriaux, PhD, Gaelle Bériou, PhD, Jérôme C. Martin, PharmD, PhD, Luc Colas, MD, Vassili Soumelis, MD, PhD, Régis Josien, MD, PhD Journal of Allergy and Clinical Immunology Volume 141, Issue 4, Pages 1476-1479.e6 (April 2018) DOI: 10.1016/j.jaci.2017.11.012 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 TSLP does not activate purified blood basophils from HDs. Blood basophils and mDCs were isolated from HDs and cultured with medium, IL-3, or TSLP for expression of CD63, CD69, and CD203c on basophils (n = 4-7) (A). Cytokine and chemokine concentration in basophil supernatants (n = 4) (B). Expression of CD83 and CD86 on mDCs (n = 6) (C). Kinetics of P-STAT5 expression in basophils (top) and mDCs (bottom) (n = 3) (D). TSLPR and IL-7Rα expression on basophils (top) and mDCs (bottom) (E). MCP-1, Monocyte chemoattractant protein; MIP-1α, macrophage inflammatory protein-1α. Data are shown as mean ± SEM. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2018 141, 1476-1479.e6DOI: (10.1016/j.jaci.2017.11.012) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Basophils from allergic patients do not respond to TSLP and do not express IL-7Rα. Whole blood samples from HDs (empty bars, n = 5) and allergic patients (black bars, n = 8) were stimulated for 6 hours with IL-3, TSLP, anti-FcɛRI antibody, or medium and analyzed by FCM. Gating strategy on whole blood (A). Intracellular P-STAT5 expression in basophils (left) and mDCs (right) (B). CD203c (left) and CCR3 (right) expression on basophils (C). % of IL-7Rα– and TSLPR- expressing basophils (left) or mDCs (right) (D). Data are shown as mean ± SEM. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology 2018 141, 1476-1479.e6DOI: (10.1016/j.jaci.2017.11.012) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 A, CD63, CD69, and CD203c expression on freshly isolated basophils and after 24-hour stimulation with medium, IL-3, or TSLP. B, CD86 and CD83 expression on isolated mDCs after 24-hour stimulation with medium or TSLP. C, Cytokine secretion by isolated mDCs after 24-hour stimulation by medium, IL-3, or TSLP and measured by a multiplex assay. MCP-1, Monocyte chemoattractant protein; MIP-1α, macrophage inflammatory protein-1α. Journal of Allergy and Clinical Immunology 2018 141, 1476-1479.e6DOI: (10.1016/j.jaci.2017.11.012) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 A, Histograms of P-STAT5 expression in purified basophils and mDCs after stimulation by IL-3 or TSLP. B, P-STAT5 expression in basophils after 24-hour priming followed by 15-minute stimulation with IL-3 or TSLP (n = 3-5 donors). Journal of Allergy and Clinical Immunology 2018 141, 1476-1479.e6DOI: (10.1016/j.jaci.2017.11.012) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Intracellular P-STAT5 expression in basophils and mDCs (A) in whole blood samples from an allergic donor, after 6-hour stimulation with IL-3, TSLP, or medium. CD203c and CCR3 expression on basophils (B) and IL-7Rα and TSLPR expression on mDCs (C) or basophils (D) in whole blood samples from an allergic donor, either fresh or after 6-hour stimulation with IL-3, TSLP, anti-FcɛRI antibody, or medium. Journal of Allergy and Clinical Immunology 2018 141, 1476-1479.e6DOI: (10.1016/j.jaci.2017.11.012) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 A, TSLPR and IL7R mRNA expression assessed by RT-PCR in fresh and IL-3–activated basophils from allergic patients (n = 3-5 per time point). B, CD203c and CD69 expression on blood basophils isolated from allergic patients using the Stem Cell Technologies kit, either fresh or after culture with medium, IL-3, TSLP, anti-IgE mAb, or anti-FcεRI mAb for 2 hours or 22 hours (n = 3-5 per time point/condition). C, TSLPR and IL-7Rα expression on blood basophils isolated from allergic patients using the Stem Cell Technologies kit, either fresh or after culture with IL-3, TSLP, anti-IgE mAb, or anti-FcεRI mAb for 2 hours or 22 hours. Similar results were obtained in 5 different allergic individuals. A.U., Arbitray unit. Journal of Allergy and Clinical Immunology 2018 141, 1476-1479.e6DOI: (10.1016/j.jaci.2017.11.012) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions