Fig. 2 Camera-based photothermal imaging.

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Presentation transcript:

Fig. 2 Camera-based photothermal imaging. Camera-based photothermal imaging. (A) Block diagram. The MCT detector was used to capture the IR laser pulses to generate the master clock f at the repetition rate of the IR laser to trigger the function generator, which sent square wave triggers to the camera, chopper, and LED. The internal frequency divider of the camera was set to expose at f/8 frames/s. The chopper divided the trigger pulses by 16 to modulate the pump. A computer was used to control the camera and store the data. (B) Measured pulses of the IR (red), visible (blue), and camera exposure monitor (purple) with an oscilloscope. For the 20-kHz laser repetition rate and 2500-Hz camera frame rate, each frame contained eight probe pulses. (C) Zoom-in view of individual pump and probe pulses. The time scale for each grid is 100 μs in (B) and 500 ns in (C). (D) Image processing procedure to generate a photothermal image. Contrast was created by subtraction between hot and cold frames. Scale bars, 40 μm. Yeran Bai et al. Sci Adv 2019;5:eaav7127 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).