GABAARs in RFMes GABAergic neurons across groups.

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GABAARs in RFMes GABAergic neurons across groups. GABAARs in RFMes GABAergic neurons across groups. Confocal microscopic images show all neurons stained for Nissl with FNS (green; A1, B1, C1), the GABAergic neurons immunostained for GABA (blue; A2, B2, C2, indicated by filled arrowhead), and for the GABAARs in single (red; A3, B3, C3, indicated by filled arrowhead) and merged images (A4, B4, C4, indicated by filled arrowhead). Note that in an SC mouse, the GABAAR immunofluorescence is minimally visible, whereas in an SD mouse, it is prominent and bright. In an SR mouse, it appeared less bright than in the SD mouse. In all cases, the immunostaining is relatively continuous though with nonuniform intensity along the plasma membrane of the GABA+ neurons. Scale bars: 20 μm. Image thickness: 500 nm in all panels. Hanieh Toossi et al. eNeuro 2017;4:ENEURO.0269-17.2017 ©2017 by Society for Neuroscience