Formation of a closed mitotic spindle occurs within the nucleus.

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Formation of a closed mitotic spindle occurs within the nucleus. Formation of a closed mitotic spindle occurs within the nucleus. Slice from an electron tomogram of a dividing budding yeast cell during prometaphase. The nuclear envelope (NE), the spindle pole bodies (SPBs), and the microtubules are clear, but, in this cell type, chromosome condensation is not sufficient to make the chromatin obvious. Scale bar, 1 µm. (Image kindly provided by Eileen O’Toole, University of Colorado, Boulder; reprinted, with permission, from McIntosh et al. 2012.)‏ J. Richard McIntosh Cold Spring Harb Perspect Biol 2016;8:a023218 ©2016 by Cold Spring Harbor Laboratory Press