Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

Slides:



Advertisements
Similar presentations
Yvan Boutin, PhD, Jacques Hébert, MD 
Advertisements

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.
Glucocorticoids inhibit chemokine generation by human eosinophils
Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4  Haifa H. Jabara, MSca, Donata Vercelli,
Vitamin D supplementation during pregnancy: Effect on the neonatal immune system in a randomized controlled trial  Eve Hornsby, PhD, Paul E. Pfeffer,
Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4  Haifa H. Jabara, MSca, Donata Vercelli,
Tan Jinquan, MD, PhD, Henrik H. Jacobi, MD, Chen Jing, MD, Claus M
Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen–specific IgE production by stimulation of IL-12 production in mice  Shinji.
The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,
The environmental pollutant pyrene induces the production of IL-4
Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M
Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G
Factors affecting the cytokine production of human T cells stimulated by different modes of activation  Yoshio Harada, MDa, Sumiko Watanabe, PhDa, Hans.
Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis  Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.
Production of chemokines and proinflammatory and antiinflammatory cytokines by human alveolar macrophages activated by IgE receptors  Philippe Gosset,
The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells  Shelby P. Umland,
Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine  Amato de Paulis,
CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgE  Ke Zhang, MD, PhD, Ling.
Estrogen enhances immunoglobulin production by human PBMCs
Differential control of TH1 versus TH2 cell responses by the combination of low-dose steroids with β2-adrenergic agonists  Elena Goleva, PhD, Annegret.
Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis  Sai Chan,
Glucocorticoids promote intrinsic human TH17 differentiation
Histamine in the immune regulation of allergic inflammation
Basophils from patients with allergic asthma show a primed phenotype
Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α  John T. Schroeder, PhD,
Β-Adrenergic regulation of the eosinophil respiratory burst as detected by lucigenin- dependent luminescence  Nicholas E. Hadjokas, PhD a,b, Joseph J.
Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice  Svetlana P. Chapoval,
Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A  Satoshi Matsukura, MD, Fumio Kokubu,
Cristiana Stellato, MD, PhDa, Jun Atsuta, MD, PhDb, Carol A
Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms  Lourdes Mozo, PhDa, Abel Gayo, BSa,
Factor analysis of asthma and atopy traits shows 2 major components, one of which is linked to markers on chromosome 5q  Catharine J. Holberg, PhD, Marilyn.
Differential immune responses to acute lower respiratory illness in early life and subsequent development of persistent wheezing and asthma  Fernando.
Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T
Increased sensitivity to bradykinin among African Americans
The effects of glucocorticoids on human eosinophils
The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells  Juha Punnonen, MD, PhD, Hans Yssel, PhD,
Chemokines induce eosinophil degranulation through CCR-3
Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization  Qutayba Hamid, MD, PhD, Editor  Journal.
IFN-τ inhibits IgE production in a murine model of allergy and in an IgE-producing human myeloma cell line  Mustafa G. Mujtaba, PhDa, Lorelie Villarete,
Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD 
IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma  Takeshi Noma, MD, PhD, Izumi.
3-O-Formyl-20R,21-epoxyresibufogenin suppresses IL-6–type cytokine actions by targeting the glycoprotein 130 subunit: Potential clinical implications 
Ganglioside GQ1b enhances Ig production by human PBMCs
Ganglioside GQ1b enhances anti–double-stranded DNA antibody and IgG production of PBMCs from patients with systemic lupus erythematosus  Naoko Kanda,
The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma  James L. Lordan,
Genetic susceptibility to food allergy is linked to differential TH2-TH1 responses in C3H/HeJ and BALB/c mice  Vivian Morafo, PhD*, Kamal Srivastava,
Case report: Streptomycin-induced anaphylactic shock during oocyte retrieval procedures for in vitro fertilization  Motoyasu Iikura, MD, PhDa, Masao Yamaguchi,
Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms  Toby J. Williams, Catherine A. Jones,
David M. Essayan, MD, Charity C
Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P
Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M
Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells  Iris Bellinghausen,
Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice  Masayuki.
Larry L. Thomas, PhD, Michelle D. Haskell, Emmanuel U
Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M
Qianli Zhuang, MD, PhD, Bruce Mazer, MD 
Chemical constituents of diesel exhaust particles induce IL-4 production and histamine release by human basophils  Gilles Devouassoux, MDa, Andrew Saxon,
CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4  Kandace Bonner,
Gp120- and TNF-α–induced modulation of human B cell function: Proliferation, cyclic AMP generation, Ig production, and B-cell receptor expression  Christina.
Pia J. Hauk, MDa, Qutayba A. Hamid, MD, PhDc, George P
Staphylococcal exotoxins exert proinflammatory effects through inhibition of eosinophil apoptosis, increased surface antigen expression (CD11b, CD45,
Sulfonamide-reactive lymphocytes detected at very low frequency in the peripheral blood of patients with drug-induced eruptions  Richard S. Kalish, MD,
Nedocromil sodium and airway inflammation in vivo and in vitro
Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy  Esther C. de Jong, MSca, b, Steven Spanhaak,
Abnormalities of cell and mediator levels in bronchoalveolar lavage fluid of patients with mild asthma  Sally E. Wenzel, MD  Journal of Allergy and Clinical.
TH2 Cytokine-enhanced and TGF-β-enhanced vascular endothelial growth factor production by cultured human airway smooth muscle cells is attenuated by IFN-γ.
Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells  Richard K.S.
Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ  Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.
Histamine release in intact human skin by monocyte chemoattractant factor-1, RANTES, macrophage inflammatory protein-1α, stem cell factor, anti-IgE, and.
CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4  Kandace Bonner,
Presentation transcript:

Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring forms of human IL-3  Andrea Schweiger, MD, Debra Stern, MS, I.Carla Lohman, MS, Mauro Baldini, MD, Fernando D. Martinez, MD, Marilyn Halonen, PhD  Journal of Allergy and Clinical Immunology  Volume 107, Issue 3, Pages 505-510 (March 2001) DOI: 10.1067/mai.2001.113239 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 The rhIL-3/P8 form of IL-3 (squares) induces enhanced proliferation of TF-1 cells compared with rhIL-3/S8 (circles) . TF-1 cells were cultured for 72 hours in triplicate. 5-Bromo-2’-deoxyuridine was added 8 hours before harvest. The number of experiments used at each concentration of IL-3 were 4 for 10–7 mol/L, 6 for 10–8 mol/L, and 7 for 10–7 mol/L and 10–12 mol/L. Absorbance units for control samples were 0.037 ± 0.006. *Significant difference in the 2 forms. All values for each form are significantly greater than control values. Journal of Allergy and Clinical Immunology 2001 107, 505-510DOI: (10.1067/mai.2001.113239) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Human leukocytes primed with rhIL-3/S8 produce greater amounts of LTs compared with rhIL-3/P8. Dextran-separated cells were primed for 24 hours and stimulated with anti-IgE for 40 minutes before harvest. Each point represents the mean fold increase in IL-3–treated samples over control values (control RPMI) ± SEM for leukocytes from 8 donors. The production of LTs was undetectable without stimulation with anti-IgE (data not shown). *Significantly different amounts of LTs for the 2 forms of IL-3. †Significant difference from control values. Journal of Allergy and Clinical Immunology 2001 107, 505-510DOI: (10.1067/mai.2001.113239) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Human leukocytes primed with rhIL-3/S8 produce greater amounts of IL-4 compared with rhIL-3/P8. Dextran-separated cells were primed for 1 (A) , 4 (B) , or 24 (C) hours and stimulated with anti-IgE for 40 minutes before harvest. Each point represents the mean fold increase over control (control RPMI) ± SEM of IL-4 production for 3 different donors. *Significant difference between the 2 forms of IL-3. †Significant difference from control values. Journal of Allergy and Clinical Immunology 2001 107, 505-510DOI: (10.1067/mai.2001.113239) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 4 IL-3 directly induces TNF-α production from human leukocytes. Cells were stimulated with (A) or without (B) anti-IgE. Each point represents the mean fold increase over control (control RPMI) ± SEM of TNF-α production for 6 donors. Values for rhIL-3/P8 were not different from control values. The data show that the production of TNF-α was not dependent on stimulation with anti-IgE (B ). *Significant differences between the 2 forms of IL-3. †Significant difference from control values. Journal of Allergy and Clinical Immunology 2001 107, 505-510DOI: (10.1067/mai.2001.113239) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 5 Polymyxin B does not inhibit the IL-3 enhancement over control of TNF-α secretion from human leukocytes. Leukocytes from one donor were stimulated with rhIL-3/S8 or rhIL-3/P8 at 10–8 mol/L in the presence or absence of polymyxin B at 10 or 20 μg and incubated for 24 hours. Journal of Allergy and Clinical Immunology 2001 107, 505-510DOI: (10.1067/mai.2001.113239) Copyright © 2001 Mosby, Inc. Terms and Conditions