Volume 27, Issue 6, Pages (March 2017)

Slides:



Advertisements
Similar presentations
Harinath Doodhi, Eugene A. Katrukha, Lukas C. Kapitein, Anna Akhmanova 
Advertisements

Transient Membrane Localization of SPV-1 Drives Cyclical Actomyosin Contractions in the C. elegans Spermatheca  Pei Yi Tan, Ronen Zaidel-Bar  Current.
Molecular Tattoo: Subcellular Confinement of Drug Effects
Harinath Doodhi, Eugene A. Katrukha, Lukas C. Kapitein, Anna Akhmanova 
Volume 147, Issue 5, Pages (November 2011)
Nuclear envelope Current Biology
Colleen T. Skau, David R. Kovar  Current Biology 
Volume 27, Issue 4, Pages (February 2017)
Dynamics of interphase microtubules in Schizosaccharomyces pombe
Volume 5, Issue 6, Pages (December 2013)
Transcriptional Memory in the Drosophila Embryo
Volume 20, Issue 24, Pages (December 2010)
Seisuke Arai, Yoichi Noda, Satoko Kainuma, Ikuo Wada, Koji Yoda 
Volume 28, Issue 5, Pages (March 2014)
Volume 25, Issue 21, Pages (November 2015)
Mutual Repression by Bantam miRNA and Capicua Links the EGFR/MAPK and Hippo Pathways in Growth Control  Héctor Herranz, Xin Hong, Stephen M. Cohen  Current.
Asymmetric Microtubule Pushing Forces in Nuclear Centering
Xianfeng Morgan Xu, Tea Meulia, Iris Meier  Current Biology 
Volume 27, Issue 18, Pages e3 (September 2017)
Volume 25, Issue 15, Pages (August 2015)
Volume 18, Issue 19, Pages (October 2008)
Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast  Jonathan M. Glynn,
Volume 148, Issue 5, Pages (March 2012)
Is Aggregate-Dependent Yeast Aging Fortuitous
Identification of Nuclear Dicing Bodies Containing Proteins for MicroRNA Biogenesis in Living Arabidopsis Plants  Yuda Fang, David L. Spector  Current.
Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic- reticulum-to-Golgi transport complexes  David T. Shima, Suzie J. Scales, Thomas.
Volume 15, Issue 5, Pages (May 2014)
Cdk1 Modulation Ensures the Coordination of Cell-Cycle Events during the Switch from Meiotic Prophase to Mitosis  Dai Tsuchiya, Soni Lacefield  Current.
Dan Zhang, Aleksandar Vjestica, Snezhana Oliferenko  Current Biology 
Volume 26, Issue 2, Pages (July 2013)
Budding Yeast Has a Minimal Endomembrane System
A Super-Assembly of Whi3 Encodes Memory of Deceptive Encounters by Single Cells during Yeast Courtship  Fabrice Caudron, Yves Barral  Cell  Volume 155,
Volume 19, Issue 20, Pages (November 2009)
Volume 22, Issue 12, Pages (June 2012)
Abdur Rahaman, Nels C. Elde, Aaron P. Turkewitz  Current Biology 
She1-Mediated Inhibition of Dynein Motility along Astral Microtubules Promotes Polarized Spindle Movements  Steven M. Markus, Katelyn A. Kalutkiewicz,
Volume 27, Issue 4, Pages (February 2017)
Wood Cell-Wall Structure Requires Local 2D-Microtubule Disassembly by a Novel Plasma Membrane-Anchored Protein  Yoshihisa Oda, Yuki Iida, Yuki Kondo,
Volume 27, Issue 16, Pages e4 (August 2017)
Jasper S. Weinberg, David G. Drubin  Current Biology 
Volume 23, Issue 24, Pages (December 2013)
Volume 25, Issue 3, Pages (February 2015)
The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin  Jennifer Landino, Ryoma.
Volume 25, Issue 20, Pages (October 2015)
Marko Kaksonen, Christopher P. Toret, David G. Drubin  Cell 
Li Xu, Anthony Bretscher  Current Biology 
Volume 147, Issue 5, Pages (November 2011)
Determinants of S. cerevisiae Dynein Localization and Activation
Volume 43, Issue 4, Pages e10 (November 2017)
Volume 2, Issue 4, Pages (October 2012)
Volume 147, Issue 5, Pages (November 2011)
Myosin-IXA Regulates Collective Epithelial Cell Migration by Targeting RhoGAP Activity to Cell-Cell Junctions  Tatiana Omelchenko, Alan Hall  Current.
Aljoscha Nern, Yan Zhu, S. Lawrence Zipursky  Neuron 
Volume 28, Issue 5, Pages (March 2014)
Volume 22, Issue 14, Pages (July 2012)
Yun Wah Lam, Angus I. Lamond, Matthias Mann, Jens S. Andersen 
Volume 26, Issue 9, Pages (May 2016)
Volume 21, Issue 2, Pages (February 2014)
Fanny S. Chang, Christopher J. Stefan, Kendall J. Blumer 
Wood Cell-Wall Structure Requires Local 2D-Microtubule Disassembly by a Novel Plasma Membrane-Anchored Protein  Yoshihisa Oda, Yuki Iida, Yuki Kondo,
Irina Chernyakov, Felipe Santiago-Tirado, Anthony Bretscher 
Volume 22, Issue 14, Pages (July 2012)
Volume 21, Issue 4, Pages (February 2011)
Contributions of Turgor Pressure, the Contractile Ring, and Septum Assembly to Forces in Cytokinesis in Fission Yeast  Stephen A. Proctor, Nicolas Minc,
Volume 23, Issue 6, Pages (May 2018)
Maria Schmid, Andreas Jaedicke, Tung-Gia Du, Ralf-Peter Jansen 
Volume 1, Issue 2, Pages (February 2012)
Cdk1 Modulation Ensures the Coordination of Cell-Cycle Events during the Switch from Meiotic Prophase to Mitosis  Dai Tsuchiya, Soni Lacefield  Current.
Divergent Strategies for Controlling the Nuclear Membrane Satisfy Geometric Constraints during Nuclear Division  Candice Yam, Yue He, Dan Zhang, Keng-Hwee.
Spa2p Functions as a Scaffold-like Protein to Recruit the Mpk1p MAP Kinase Module to Sites of Polarized Growth  Frank van Drogen, Matthias Peter  Current.
Presentation transcript:

Volume 27, Issue 6, Pages 773-783 (March 2017) Compartmentalization of ER-Bound Chaperone Confines Protein Deposit Formation to the Aging Yeast Cell  Juha Saarikangas, Fabrice Caudron, Rupali Prasad, David F. Moreno, Alessio Bolognesi, Martí Aldea, Yves Barral  Current Biology  Volume 27, Issue 6, Pages 773-783 (March 2017) DOI: 10.1016/j.cub.2017.01.069 Copyright © 2017 Elsevier Ltd Terms and Conditions

Current Biology 2017 27, 773-783DOI: (10.1016/j.cub.2017.01.069) Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 1 Age-Dependent Deposits Associate with the Endoplasmic Reticulum (A) Representative 3D projected image of a cell expressing Sec61-GFP and Hsp104-mCherry. (B) The percentages of age-associated protein deposits (Hsp104-mCherry focus) in contact with different organelles (n = 18–51/group). (C) Time-lapse imaging of budding cell expressing Hsp104-mCherry and Sec61-GFP. Arrowhead, deposit surrounded by an ER extension. (D) Quantification of the apposition of Hsp104-mCherry-labeled deposits to the ER (Sec71-GFP), vacuole (Ym018c-GFP), and mitochondria (Tom70-GFP) during cell division. Images were acquired every 10 min over 100 min. 0 min time point is set at G1 (n = 15/each). (E) Design of the mating experiments. (F and G) Time-lapse images at indicated time points after conjugation. MATa cell expresses Hsp104-mCherry (arrowhead, deposit) and Sec71-GFP (ER membrane marker) (F); Matα cell expresses Hsp104-mCherry or Yml018c-GFP (vacuolar membrane) (G). (H) Quantification of apposition between labeled deposit (Hsp104-mCherry) and the ER (Sec71-GFP) or vacuole (Ymo018c) starting from first frame before fusion. (I and J) Single-plane SIM images of cells expressing Sec71-GFP and Hsp104-mCherry. Arrowheads, deposits at the nuclear envelope (I) and inside the nucleus (J). Scale bars represent 2 μm (A), 5 μm (C, F, and G), and 1 μm (I and J). See also Figures S1 and S2. Current Biology 2017 27, 773-783DOI: (10.1016/j.cub.2017.01.069) Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 2 Localization to the ER Membrane Promotes the Singularity of Age-Associated Deposits (A) Time-lapse imaging of Hsp104-GFP in a middle-age mother cell (12–15 generations). Arrowheads, pre-existing age-associated deposit (red) and transient appearance of a second one (blue). (B–G) The experimental principles (B) and representative 3D projected images captured with time-lapse microscopy at indicated time points of a MATa cell expressing Hsp104-GFP (Mata) fusing with a MATα cell expressing Hsp104-mCherry (C–G). The fluorescence intensity line profiles depicted in the lower panel span across the fusing cells crossing the site of aggregates (intensity peaks). 0 min corresponds to time of cell-cell fusion. (H) Deletion of PRM3 impairs nuclear and ER fusion [22]. (I and J) Representative images at indicated time points after fusion of wild-type zygotes (I) and prm3Δ/prm3Δ mutant zygotes (J) expressing Hsp104-mCherry (gray) and Pre6-GFP (blue, nucleus). (K) Graph explains how deposit fusion events were quantified. (L) Quantification of wild-type and prm3Δ/prm3Δ mutant zygotes fusing deposits before completing their first division. Graph displays mean in % ± SEM (n = 42–81). Scale bars represent 5 μm. See also Figure S3 and Movie S1. Current Biology 2017 27, 773-783DOI: (10.1016/j.cub.2017.01.069) Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 3 Lateral Diffusion Barriers at the Endoplasmic Reticulum Inhibit Deposit Formation in the Daughter Cells (A) Representative projections of wild-type, bud1Δ, bud6Δ, and sur2Δ mutant cells expressing Hsp104-GFP (gray) imaged 285 min apart. Red arrowheads, deposit in the aged mother cell (red) and deposits appearing in the daughter cells (blue). (B) Percentage of divisions in which the deposit passes to the daughter cells (from aged mother cell with a single deposit) in strains of indicated genotype (n = 117–269). (C) Percentage of daughter cells forming a stable deposit during their first cell cycle as in (B) (n = 82–244). The graphs display mean ± SEM. Scale bars represent 5 μm. See also Figure S4. Current Biology 2017 27, 773-783DOI: (10.1016/j.cub.2017.01.069) Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 4 Farnesylated Ydj1 and the Diffusion Barriers Confine Protein Deposit Formation to the Mother Cell (A and B) Representative projections of wild-type (A) and Ydj1-C406S mutant cells (B) imaged 120 min apart as in Figure 3A. Arrowheads, deposits of the mother cell (red) and deposits appearing in the daughter cells (blue). D, daughter; GD, granddaughter cell; M, mother. (C) Percentage of divisions with deposit passing from the mother cell into her daughter (n = 217–502). (D) Percentage of daughter cells forming a stable deposit during their first division cycle (as in Figure 1C; n = 318–343). (E) Representative images of YDJ1-C406S sur2Δ, YDJ1-C406S bud1Δ, and YDJ1-C406S bud6Δ double-mutant cells as in (A). (F) Percentage of daughter cells forming a deposit during their first division cycle (n = 256–343). Scale bars represent 5 μm; graphs display mean ± SEM. See also Figure S5. Current Biology 2017 27, 773-783DOI: (10.1016/j.cub.2017.01.069) Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 5 Ydj1 Is Compartmentalized to the Mother Cells by ER Tethering and Diffusion Barriers, and Its ER Anchoring Suppresses the Sup35 Prion Phenotype (A) Sketch of the experimental design. (B and C) Quantification of the diffusion coefficient presented (in log scale) in the indicated compartments (B), or presented as a mother/bud ratio of GFP (n = 53), Ydj1-GFPFS (n = 149), Ydj1-GFPC406S (n = 48), and Ydj1-GFPFS in sur2Δ cells (n = 98) (C). (D) Schematic representation of the FLIP assay: GFP-Ydj1 was continuously photobleached in the mother domain, and the fluorescence decay was measured over time in mother (red) and bud (orange). (E) Average FLIP curves of GFP-Ydj1 (upper panel; n = 40); Ydj1-GFP (lower panel; n = 39). (F) Barrier index for the indicated markers in cells of indicated genotypes. (G) Schematic representation of the effects of Sup35 in translation termination in its non-prion [psi−] and weak- and strong-prion [PSI+] states. (H) Fluorescence intensity measured by flow cytometer of YDJ1, YDJ1-C406S, and Ydj1-L135S cells in the [PSI+] and [psi−] states. Graphs display mean ± SEM. Current Biology 2017 27, 773-783DOI: (10.1016/j.cub.2017.01.069) Copyright © 2017 Elsevier Ltd Terms and Conditions