Targeting endothelial junctional adhesion molecule‐A/ EPAC/ Rap‐1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles JAM‐A‐WT.

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Targeting endothelial junctional adhesion molecule‐A/ EPAC/ Rap‐1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles JAM‐A‐WT endothelial cells were starved for 16 h and then treated with vehicle (ctrl), a selective activator of EPAC (007, 100 μM) or tetradecanoylphorbol acetate (TPA, 10 μM), an activator of the signal transduction enzyme protein kinase C, used as positive control for Rap‐1 activation. Rap1‐GTP loading was quantified by GST pull‐down/ Western blotting and densitometry analysis. Representative blot (left) and quantification of densitometry analysis (right) of Rap‐1‐GTP levels normalized for total amount of protein. **P < 0.001. Data are means ± s.d. from three independent experiments. JAM‐A‐WT endothelial cells were incubated with the Rap‐1 pharmacological inhibitor GGTI‐298 (10 μM) or vehicle for 3 h and 6 h (as indicated). Rap1‐GTP loading was quantified as in A. Data are means ± s.d. from three independent experiments. Note: to highlight Rap‐1 activation, the filter in A was exposed for 30 s, while in B it was exposed for 5 min, to improve the evaluation of Rap‐1 inhibition at 3 h and 6 h. JAM‐A‐WT and JAM‐A‐null endothelial cells seeded on coated filters for 72 h and treated with vehicle (ctrl) or 007 or GGTI‐298 for the last 30 min, as indicated. 6‐CFDA‐labelled adult MABs were added to the upper chamber and allowed to migrate for 6 h under these conditions. Quantification of migrated MABs per area for JAM‐A‐WT (on the left of the dashed line) and JAM‐A‐null (on the right of the dashed line) endothelial cells. **P < 0.001. Data are means ± s.e.m. from three independent experiments, each in triplicate. 6‐CFDA‐labelled embryonic (left) and adult (right) MABs were incubated with vehicle (ctrl, white bars), 007 (100 μM) or GGTI‐298 (10 μM) (black bars) for 3 h, as indicated. Then the MABs were seeded on filters and allowed to migrate for a further 3 h in the presence of the indicated agents. The migrated MABs on the lower side of the filters (green) were fixed and counted. Quantification of migrated cells per area is shown. Data are means ± s.e.m. from three independent experiments, each in triplicate. HUVECs were treated as described in C. Quantification of migrated MABs per area is shown for 22 y.o. (left), 42 y.o. (middle) and 37 y.o. (right) MABs. **P < 0.001. Data are means ± s.e.m. from three independent experiments, each in triplicate. Sgca‐null mice were treated with GGTI‐298 (50 mg/Kg, n = 2) or with vehicle (ctrl, n = 3) for 1 h and then were intra‐arterially transplanted with adult MABs. After 6 h, the hind limb muscles were collected and the presence of migrated cells was quantified using qRT‐PCR with nLacZ primers. The relative RNA level of nLacZ obtained for control was set to 1, and the ratio for GGTI‐298 versus control is shown. Fold increases have been extrapolated by data shown in Figure S1H. Box‐plot showing exercise tolerance in a treadmill test for untreated Sgca‐null/scid/beige mice, Sgca‐null/scid/beige mice transplanted with MABs (+ MABs, 5 × 105 cells injected bilaterally in femoral arteries) and Sgca‐null/scid/beige mice transplanted with MABs and treated with GGTI‐298 (+MABs + GGTI‐298, 5 × 105 cells injected bilaterally in femoral arteries). Values are plotted as fold increase of motor capacity measured at different time points (21, 28 and 35 days post transplantation), and were normalized with the baseline performances of each mouse. Data are means ± s.e.m. of 3 mice per group. **P < 0.01, ***P < 0.001, one‐way ANOVA. Source data are available for this figure. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO Mol Med, Volume: 6, Issue: 2, Pages: 239-258, First published: 30 December 2013, DOI: (10.1002/emmm.201302520)