An ipRGC axon collateral colocalized with the presynaptic marker bassoon makes contact with TH-positive DAC processes. An ipRGC axon collateral colocalized.

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An ipRGC axon collateral colocalized with the presynaptic marker bassoon makes contact with TH-positive DAC processes. An ipRGC axon collateral colocalized with the presynaptic marker bassoon makes contact with TH-positive DAC processes. A, Confocal images taken in 2 separate 1 μm optical sections within the same z-stack from a whole-mount retina in which DAC processes are marked by TH (blue), presynaptic active zones are labeled by bassoon (green), and an ipRGC axon collateral is labeled by alkaline phosphatase (red). Arrows indicate the colocalization of the presynaptic marker and the ipRGC axon collateral in direct apposition to DAC dendrites. Scale bar, 5 μm. B, Average number and cellular localization of bassoon-positive puncta at sites of contact between ipRGC axon collaterals and DACs (left bars) and ipRGC dendrites and DACs (right bars). **p < 0.01, Tukey's test (n = 3). Cameron L. Prigge et al. J. Neurosci. 2016;36:7184-7197 ©2016 by Society for Neuroscience