Fig. 5. Differentiation assay of fluorescence-activated cell sorter-sorted platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells. (A) In.

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Fig. 5. Differentiation assay of fluorescence-activated cell sorter-sorted platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells. (A) In osteogenic differentiation, which was assayed with alkaline phosphatase (ALP) staining, the PDGFRβ+ cells showed greater ALP staining. (B) In chondrogenic differentiation, which was verified by Safranin O staining, the PDGFRβ+ cells showed greater chondrogenic differentiation. (C) After adipogenic induction for 3 days, the cell morphology changed from long spindle-shape into a round or polygonal shape. One week later, small bubble-shaped oil red O-staining lipid droplets appeared in part of the cells. ADSC: adipose-derived stem cells. Fig. 5. Differentiation assay of fluorescence-activated cell sorter-sorted platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells. (A) In osteogenic differentiation, which was assayed with alkaline phosphatase (ALP) staining, the PDGFRβ+ cells showed greater ALP staining. (B) In chondrogenic differentiation, which was verified by Safranin O staining, the… Clin Orthop Surg. 2017 Jun;9(2):239-248. https://doi.org/10.4055/cios.2017.9.2.239