Phosphoinositide Signaling Glenn D Prestwich  Chemistry & Biology  Volume 11, Issue 5, Pages 619-637 (May 2004) DOI: 10.1016/j.chembiol.2004.03.025

Figure 1 Chemistry of Phosphoinositide Metabolism (A) PI 3-K reaction. (B) Interconversions of PIPns by lipid kinases (red) and lipid phosphatases (green). Enzyme abbreviations are defined in the text. (C) Structures of the phosphatidylinositol polyphosphates and synthetic analogs. Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)

Figure 2 The PI 3-K/Akt Signaling Pathway and Potential Drug Targets for Cancer Therapy Additional details on therapeutic targets are provided in several reviews [49–51, 55]. Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)

Figure 3 Molecular Topologies of Different Tethering Modes for InsPn and PIPn Affinity Probes Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)

Figure 4 Chemistry of Tethered Phosphoinositide Topologies (A) Comparison of PI(4,5)P2 molecular types attached to a resin, lipophilic BODIPY fluorophore, biotin, benzoyldihydrocinnamoyl (BZDC) photophore, and proxyl spin label. (B) Preparation of a PI(3,4,5)P3 PolyPIPosome by irradiation of a polymerizable liposome containing a diyne-linked Ins(1,3,4,5)P4 head group. Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)

Figure 5 Immunodetection of Phosphoinositides KLH, keyhole limpet hemocyanin; EDCI, ethyl N,N-dimethylaminopropylcarbodiimide. (A) Preparation of an antigenic PI(3,4,5)P3 bioconjugate. (B) Visualization of PI(3,4,5)P3 in stimulated fibroblasts [171]. Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)

Figure 6 Confocal Images Illustrating the Uptake of NBD-PI(3,4)P2 and Texas Red Histone in NIH 3T3 Fibroblasts Using Intracellular Delivery Methods (A) Sequential optical z-sections (0.2 μm) were collected 5 min after addition of PI(3,4)P2-histone complex. The montage shows six successive 1.5 μm steps from the top of the cell (1) to the attachment of the cell to the substratum (6). (B) Pseudo-three-dimensional reconstruction. Images are courtesy of J. Shope and D.B. DeWald [107]. Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)

Figure 7 Drug Discovery in the PI 3-K Pathway (A) Fluorescence polarization assay for PI 3-K [114]. (B) Selected inhibitors for PI 3-K [50]. (C) Akt PH domain antagonists [56]. Chemistry & Biology 2004 11, 619-637DOI: (10.1016/j.chembiol.2004.03.025)