Figure 2. Microscopic evaluation and 11β-HSD 1 activity in cells grown under monolayer or spheroid culture conditions. (A) Cells were grown in monolayer.

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Figure 2. Microscopic evaluation and 11β-HSD 1 activity in cells grown under monolayer or spheroid culture conditions. (A) Cells were grown in monolayer or spheroid conditions and then observed microscopically using 10× ocular lens and 2× objective lens. (B) Cortisol levels in culture medium from SW982 cells grown under different conditions were measured. Cells were incubated in DMEM containing 160 nM cortisone as a substrate of 11β-HSD1. Figure 2. Microscopic evaluation and 11β-HSD 1 activity in cells grown under monolayer or spheroid culture conditions. (A) Cells were grown in monolayer or spheroid conditions and then observed microscopically using 10× ocular lens and 2× objective lens. (B) Cortisol levels in culture medium from SW982 cells grown under different… Immune Netw. 2017 Jun;17(3):171-178. https://doi.org/10.4110/in.2017.17.3.171