A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis.

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Presentation transcript:

A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis. A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis. Columns, mean (n = 3) in a representative experiment; bars, SE. B, IGFBP-3 down-regulation via siRNA using Western immunoblot analysis. β-Actin was used as loading control. Lane 1, mismatch control for IGFBP-3; lane 2, transfection reagent control; lane 3, siRNA at 100 nmol/L; lane 4, siRNA at 200 nmol/L; lane 5, siRNA at 400 nmol/L. Yaguang Xi et al. Mol Cancer Ther 2006;5:3078-3084 ©2006 by American Association for Cancer Research