Activating mutation of Ras is associated with CDCP1 expression in NSCLC. A, NSCLC cell lines with Ras + B-Raf mutations and wild-type Ras were examined by qRT-PCR and compared with normal lung. Activating mutation of Ras is associated with CDCP1 expression in NSCLC. A, NSCLC cell lines with Ras + B-Raf mutations and wild-type Ras were examined by qRT-PCR and compared with normal lung. B, expression of CDCP1, ERK1/2, and phosphorylation of ERK1/2 in each cell line. Black arrowheads denote band corresponding to CDCP1. White arrowhead indicates nonspecific bands. C, the expression of CDCP1 and the ratio of phosphorylated ERK in each cell line. D, human lung cancer tissues with mutations of Ras and wild-type Ras were stained by CDCP1 antibody. Magnification, ×200. E, CDCP1 expression in human lung cancer tissues with mutations of Ras compared with wild-type Ras. P values in a Student t test = 0.009. Takamasa Uekita et al. Mol Cancer Res 2014;12:1449-1459 ©2014 by American Association for Cancer Research