Results from human serum samples determined using the five-antigen multiplex assay. Results from human serum samples determined using the five-antigen multiplex assay. The MFI values (y axis) of antibodies to each particular antigen are indicated by colored bars. The patient categories are indicated below the x axis, according to the following key: healthy, from an area of endemicity (HE); healthy, from an area of nonendemicity (HNE); fibromyalgia (FB); infectious mononucleosis (MN); multiple sclerosis (MS); rheumatoid arthritis (RA); severe periodontitis (PD); syphilis (SY); early Lyme/acute phase (EL/A); early Lyme convalescent phase (EL/C); Lyme arthritis (LA); and neuroborreliosis (NL). Each sample indicated on the x axis represents an individual patient. Monica E. Embers et al. Clin. Vaccine Immunol. 2016; doi:10.1128/CVI.00685-15