The WD40 domain of ATG16L1 is required for its non‐canonical role in lipidation of LC3 at single membranes Western blotting of LC3 in ATG16L1 FL, ΔFBD‐

Slides:

Advertisements

Similar presentations

Individualized pain management plan.

Advertisements

The WD domain of ATG16L1 is not required for canonical autophagy

Isotope‐based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation Reach‐scale conceptual model of the fluvial carbon.

Isotope‐based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation Modeling domain for the main stem of the South Elkhorn.

(a) illuminance measured in the horizontal plane from a typical street light (Phillips Cosmopolis, metal halide lamp). The intensity of light decays rapidly.

Isotope‐based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation Sensitivity analysis of the ISOFLOC model displays.

Isotope‐based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation Model flowchart for ISOFLOC which details inputs,

Evening evapotranspirative cooling in relation to vegetation and urban geometry in the city of Ouagadougou, Burkina Faso Relation between sky view factor.

Matrilin‐2 prevents the TNFα‐induced apoptosis of WB‐F344 cells via suppressing JNK pathway Matrilin‐2 knockdown promotes the apoptosis of WB‐F344 cells.

Regulation of cargo‐selective endocytosis by dynamin 2 GTPase‐activating protein girdin AThe endocytic sites for the indicated cargoes were investigated.

KLF4 is a key determinant in the development and progression of cerebral cavernous malformations qRT–PCR analysis of Klf4 during disease progression at.

Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment Spinning disk confocal microscopy images showing spindle region magnifications.

Details of the binding sites of tetracycline and edeine

Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α Protein immunoblotting of cell extracts and ConA precipitates.

The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication Representative images of wt and mdi1 ovarioles stained.

Co‐immunoprecipitation of Larp with MDI‐myc in transfected S2 cells

Allelic exchange at the endogenous genomic locus in Plasmodium falciparum proves the role of dihydropteroate synthase in sulfadoxine‐resistant malaria.

Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries Global Maps Presenting the Most.

The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes The stable MDA‐MB‐231 cell line expressing FLAG‐HaloTag‐WASH (FHT‐WASH)

The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes MDA‐MB‐231 cells expressing HaloTag WASH or CCDC53 were stained with.

Regional weather and climates of the British Isles ‐ Part 1: Introduction The annual rainfall anomaly (percentage difference from normal) for Western Scotland.

Progerin accelerates atherosclerosis by inducing endoplasmic reticulum stress in vascular smooth muscle cells A, BRepresentative immunofluorescence images.

Applications of genome‐scale metabolic reconstructions

Representative electron density maps

Endosome and Golgi‐associated degradation (EGAD) of membrane proteins regulates sphingolipid metabolism Equal amounts of WT cells and indicated single.

Retinoids activate proton transport by the uncoupling proteins UCP1 and UCP2 (A) pH dependency of the state 4 respiratory rate: control ▵ and UCP2 mitochondria.

Anthropogenic noise weakens territorial response to intruder's songs

Chk1 and 14‐3‐3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest Experimental procedures of the SILAC assay to identify binding.

The mutant p53‐ID4 complex controls VEGFA isoforms by recruiting lncRNA MALAT1 AWestern blot analysis of MDA‐MB‐468 cells interfered or not for hnRNP A1.

Prevalence of women with advanced maternal age by country.

Unanchored tri‐NEDD8 inhibits PARP‐1 to protect from oxidative stress‐induced cell death WT and NEDP1 KO HEK 293 cells were transfected with FLAG‐NSUN2.

Model for end‐stage liver disease exception points for treatment‐responsive hepatocellular carcinoma Treatment‐responsive HCC: patient with complete response.

Taurine as a constituent of mitochondrial tRNAs: new insights into the functions of taurine and human mitochondrial diseases Schematic depiction of modification.

Miro proteins coordinate microtubule‐ and actin‐dependent mitochondrial transport and distribution A, BMyo19 levels correlate with the quantity of Miro.

CNOT6L couples the selective degradation of maternal transcripts to meiotic cell cycle progression in mouse oocyte AHematoxylin and eosin staining showing.

Sequential treatment with 5‐aza‐2′‐deoxycytidine and deacetylase inhibitors reactivates HIV‐1 One night after cell purification, CD8+‐depleted PBMCs from.

Induced chromosome deletions cause hypersociability and other features of Williams–Beuren syndrome in mice Expression of genes measured by real‐time qRT‐PCR.

Cyclin D2 protein is expressed in the developing human cortex

Structural and functional analysis of the GABARAP interaction motif (GIM) Interaction profile of 30 biotinylated LIR peptides from various proteins against.

APhagocytosis assay of apoptotic or necrotic GFP‐expressing RAW264

Endosome and Golgi‐associated degradation (EGAD) of membrane proteins regulates sphingolipid metabolism See also Fig EV3. Epifluorescence and phase‐contrast.

Absolute quantification of translational regulation and burden using combined sequencing approaches Genetic design of the LacZ reporter construct whose.

Mammary stem cells and progenitors: targeting the roots of breast cancer for prevention (A) Schematic of the human breast highlighting terminal ductal.

Chk1 and 14‐3‐3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest ASchematic overview of the experimental setting and immunoblot.

Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae

Structural and functional analysis of the GABARAP interaction motif (GIM) GFP‐mCherry‐p62/SQSTM1 WT, T339V, L341V and T339V/L341V were overexpressed in.

Involvement of Crm1 in the hemin‐induced nuclear exclusion of Bach1

Attenuation of cGAS‐STING signaling is mediated by a p62/SQSTM1‐dependent autophagy pathway activated by TBK1 AControl and TBK1 KO THP1 cells were stimulated.

GSTZ1‐1 Deficiency Activates NRF2/IGF1R Axis in HCC via Accumulation of Oncometabolite Succinylacetone AGSTZ1 mRNA expression in HCC and paired non‐tumor.

Heterogeneity in Δψmit reported by TMRE

Mitochondria are morphologically and functionally heterogeneous within cells Surface‐rendered three‐dimensional reconstructions of cells expressing mito‐DsRed1.

Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis Immunostaining of HeLa cells probed for FITC‐labeled streptavidin.

Confocal images representing the localization of PCDH7

Unanchored tri‐NEDD8 inhibits PARP‐1 to protect from oxidative stress‐induced cell death Western blot analysis of whole‐cell lysates from U2OS WT and NEDP1.

Cesarean section scar diverticulum evaluation by saline contrast‐enhanced magnetic resonance imaging: The relationship between variable parameters and.

Schematic diagram of MAPKKK5 constructs

M‐LDH serves as a sarcolemmal KATP channel subunit essential for cell protection against ischemia The opening of KATP channels and intact activity of M‐LDH.

GSTZ1‐1 Deficiency Activates NRF2/IGF1R Axis in HCC via Accumulation of Oncometabolite Succinylacetone AWestern blotting shows endogenous GSTZ1‐1 protein.

Repeat‐associated non‐AUG translation in C9orf72‐ALS/FTD is driven by neuronal excitation and stress AFilter‐trap binding assays show that the ISR proteins.

2c18 expression levels influence γ1‐adaptin membrane association

STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells IL‐3‐induced apoptosis in Ba/F3 cells expressing.

Processive movement of single kinesins on crowded microtubules visualized using quantum dots Effect of the mutant E164A on the motility of single quantum.

EglN2 associates with the NRF1‐PGC1α complex and controls mitochondrial function in breast cancer A, BqRT–PCR of mRNA from T47D transfected with either.

Imaging Disaster: Puerto Rico through the Eye of Hurricane María

CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression Immunoprecipitation of CD147 from RBL.

CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression The transmembrane and cytoplasmic regions.

Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy ATG16L1−/− cells reconstituted with ATG16L1WT or ATG16L1LE.

Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy Schematic presentation of ATG16L1 fragments and mutants.

(A) Effects of EGCG inhibitor at short term in N, T, and R cell lines

The heterotetrameric architecture of the epithelial sodium channel (ENaC) ENaC expressed at the cell surface is composed of a fixed number of α, β and.

Presentation transcript:

The WD40 domain of ATG16L1 is required for its non‐canonical role in lipidation of LC3 at single membranes Western blotting of LC3 in ATG16L1 FL, ΔFBD‐ or ΔWD‐complemented HCT116 cells treated with bafilomycin (100 nM) or monensin (100 μM) for 1 h. Below is the quantification of LC3‐II/LC3‐I ratios. Western blotting of LC3 from ATG16L1‐complemented HCT116 cells treated with wortmannin (67 μM), monensin (100 μM) or both for 1 h. Below is the quantification of LC3‐II/LC3‐I ratios. Confocal images of latex bead‐containing phagosomes in control and monensin‐treated GFP‐LC3‐expressing HCT116 cells complemented with ATG16L1 FL, ΔFBD or ΔWD. Samples were stained for LAMP1. Cropped images show bead phagosomes. Scale bar: 5 μm. Quantification of GFP‐LC3 recruitment to LAMP‐1‐positive phagosomes. 100 phagosomes were counted per experiment. Data information: In (A, B, D), data are presented as mean ± SEM from three separate experiments. (A) *P < 0.02, ***P < 0.001. (B) **P < 0.0002 (Student's t‐test). IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 37, Issue: 4, First published: 09 January 2018, DOI: (10.15252/embj.201797840)