Bone marrow clonal T-cell population in lymphomatoid papulosis Mitsuhito Ota, MD, Daisuke Sawamura, MD, PhD, Akihiko Shibaki, MD, PhD, Hiroshi Shimizu, MD, PhD Journal of the American Academy of Dermatology Volume 52, Issue 4, Pages 710-711 (April 2005) DOI: 10.1016/j.jaad.2004.12.022 Copyright © 2005 American Academy of Dermatology, Inc. Terms and Conditions
Fig 1 Analysis of the PCR products on a polyacrylamide gel. Each PCR reaction contained the following primers: Vβ1 (5′-TGT Ac/tC TCT GTG CCA GCA G-3′); Vβ2 (5′-TGT Ac/tC TCT GCA GTG CTA G-3′); Dβ1 (5′-CAA AGC TGT AAC ATT GTG GGG AC-3′); Dβ2 (5′-TCA TGG TGT AAC ATT GTG GGG AC-3′); Jβ1 (5′-ACA GTG AGC Ct/gG GTc/t CC-3′); and Jβ2 (5′-AGC ACt/c/g GTG AGC Ct/gG GTG CC-3′) to amplify TCR-β D-N-J fragments or V-N′-D-N-J fragments simultaneously. The arrow shows one clonally rearranged band in the skin lesion and the identical band within a smear background in the bone marrow sample. M, Size marker; Cs, skin negative control; CBM, bone marrow negative control. Journal of the American Academy of Dermatology 2005 52, 710-711DOI: (10.1016/j.jaad.2004.12.022) Copyright © 2005 American Academy of Dermatology, Inc. Terms and Conditions