Agarose gel electrophoresis of ribosomal RNA gene polymerase chain reaction (PCR) products using Borrelia afzelii (top) and B burgdorferi sensu stricto.

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Presentation transcript:

Agarose gel electrophoresis of ribosomal RNA gene polymerase chain reaction (PCR) products using Borrelia afzelii (top) and B burgdorferi sensu stricto (bottom) specific primers. Agarose gel electrophoresis of ribosomal RNA gene polymerase chain reaction (PCR) products using Borrelia afzelii (top) and B burgdorferi sensu stricto (bottom) specific primers. Reference strain B afzelii ACA-1 (lane 2) and tick isolates C7, E5, F2, F8, and J1 (lanes 6, 7, 11, 12, and 17, respectively) were B afzelii positive; reference strains B garinii A57 (lane 3) and B garinii A60 (lane 5) were negative; and reference strain B burgdorferi sensu stricto HB1 (lane 4) and tick isolates E8, E9, E10, G4, G5, G10, and H1 (lanes 8, 9, 10, 13,14 15, and 16, respectively) were B burgdorferi sensu stricto positive. Lanes 1 and 18 contain a 123 bp ladder. C L Ling et al. Mol Path 2000;53:94-98 ©2000 by BMJ Publishing Group Ltd and Association of Clinical Pathologists