Yukihiro Terada, M. D. , Sou-Ichi Nakamura, M. D. , Laura Hewitson, Ph

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Presentation transcript:

Human sperm aster formation after intracytoplasmic sperm injection with rabbit and bovine eggs Yukihiro Terada, M.D., Sou-Ichi Nakamura, M.D., Laura Hewitson, Ph.D., Calvin Simerly, Ph.D., Toshitaka Horiuchi, M.D., Takashi Murakami, M.D., Kunihiro Okamura, M.D., Gerald Schatten, Ph.D. Fertility and Sterility Volume 77, Issue 6, Pages 1283-1284 (June 2002) DOI: 10.1016/S0015-0282(02)03106-0

FIGURE 1 Human sperm aster formation in vivo after ICSI into rabbit (A) and bovine (B) oocytes. (A), Formation of the human sperm aster after heterologous ICSI into rabbit eggs. Human sperm from fertile donors were microinjected into matured rabbit eggs. Eggs were fixed 3 hours after ICSI and stained by using the immunofluorescent method against tubulin (green) and DNA (blue). The sperm aster was organized from the sperm centrosome (arrow). The arrowhead indicates the sperm tail. (B), Formation of the human sperm aster in bovine eggs after ICSI by Piezo micromanipulator. Six hours after ICSI, the sperm aster was organized from the sperm centrosome (arrow). The arrowhead indicates the sperm tail. Terada. Human sperm aster formation after ICSI. Fertil Steril 2002. Fertility and Sterility 2002 77, 1283-1284DOI: (10.1016/S0015-0282(02)03106-0)