RNAi knockdown reveals requirement for Sb, CG16884, and hui in wing development (A) Wild-type wing, with longitudinal veins L1-5, anterior (acv) and posterior.

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RNAi knockdown reveals requirement for Sb, CG16884, and hui in wing development (A) Wild-type wing, with longitudinal veins L1-5, anterior (acv) and posterior crossveins (pcv) and the A/P compartment border indicated. RNAi knockdown reveals requirement for Sb, CG16884, and hui in wing development (A) Wild-type wing, with longitudinal veins L1-5, anterior (acv) and posterior crossveins (pcv) and the A/P compartment border indicated. (B) RNAi constructs directed against hui, Sb, and CG16884 (columns) were expressed along the A/P border (ptc-Gal4, top row), throughout the disc (C765-Gal4, middle row), or in the P compartment (en-Gal4, third row). hui-RNAi driven by ptc-Gal4 resulted in stubby wings with narrowed L3-L4 intervein region and missing acv (indicative of Hh signaling deficits, arrow). When driven by C765-Gal4 and en-Gal4, these wings have abnormal shape and venation. Phasing of the bristles at the posterior wing margin was disturbed following activation with en-Gal4 (arrow in detail view showing a comparison to a wild-type margin). Expression of Sb RNAi under ptc-Gal4 reduced or eliminated the acv (arrow in top left and detail view bottom row, compare to control in (A). Expression of Sb RNAi under C765-Gal4 reduced the size of both A and P compartments and caused some ectopic vein formation. Sb RNAi in P cells (en-Gal4) caused no apparent effect. CG16884-RNAi in the ptc domain caused narrowing of the L3-L4 intervein region. Knock-down with C765-Gal4 resulted in ectopic vein formation (arrow); RNAi in P cells caused size reduction of the P compartment and a fragile and less pigmented appearance of the cuticle (detail view). All images except for the detail views are to scale. Daniel M. Ibrahim et al. G3 2013;3:1353-1362 ©2013 by Genetics Society of America