PTEN inhibition unmasks the antihyperalgesic efficacy of the δR agonist SNC80. PTEN inhibition unmasks the antihyperalgesic efficacy of the δR agonist.

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PTEN inhibition unmasks the antihyperalgesic efficacy of the δR agonist SNC80. PTEN inhibition unmasks the antihyperalgesic efficacy of the δR agonist SNC80. A, The threshold for mechanical hyperalgesia was determined using a manual von Frey hair, in mice after intraplantar injection of CFA. A schematic shows the timeline and doses used in our experiments. B, Mechanical thresholds in mice show that pretreatment with PTEN inhibitor unmasked the antihyperalgesic effects of SNC80 (mean ± SEM). **p < 0.01. SNC80 or bpv (Phen) on their own did not show antihyperalgesia compared with control vehicle-injected mice. The mechanical threshold before CFA treatment was used as baseline (green dashed line). C, A schematic showing the timeline and doses used in testing whether naltrindole blocks the bpV(Phen)-mediated increase in SNC80 efficacy. D, Naltrindole abolishes the increase in SNC80-mediated antihyperalgesia caused by PTEN inhibition (mean ± SEM). **p < 0.01. E, Proposed model for how PTEN/PI3K balance provides a Golgi checkpoint to regulate δR export and surface availability. Following neuronal signals, such as NGF, δR is retained within the TGN due to disruption of the 3′ phosphoinositide balance by either inhibiting PI3K or increasing PTEN activity. By blocking PTEN activity via pharmacologic inhibition, 3′ phosphorylation of phosphoinositides is maintained and δR surface trafficking is stimulated. Increased δR surface trafficking via PTEN inhibition results in greater receptor surface localization, increased agonist-induced δR function, and unmasks the antihyperalgesic effects of SNC80. Daniel J. Shiwarski et al. J. Neurosci. 2017;37:3741-3752 ©2017 by Society for Neuroscience