Absorbance (600 nm) Time (h)

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Time (hours) O.D. at 600 nm w.t.ANA-3 on glucose w.t.ANA-3 on lactate w.t.MR-1 on glucose.

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Absorbance (600 nm) Time (h) 1 0.1 0.01 5 10 15 20 25 30 5 10 15 20 25 30 Time (h) Figure S1. Effects of metD (abc-yaeE-yaeC) operon expression in trans on growth of the wild type, the abc-yaeE mutant and the yaeC mutant in M9 minimal medium as described in Figure 1. Ampicillin and arabinose were added to the medium at 100 g/ml and 2 mM, respectively. , BW25113 (pBAD24); , BW25113 (pBAD24-metD); , abc-yaeE (pBAD24); , abc -yaeE (pBAD24-metD); , yaeC (pBAD24); and , yaeC (pBAD24-metD).