Representative Western blot analyses of S-phase and other proteins from PC3 cell lysates obtained 3 days after oligomer (400 nm)/Lipofectin (15 μg/ml) treatment. Representative Western blot analyses of S-phase and other proteins from PC3 cell lysates obtained 3 days after oligomer (400 nm)/Lipofectin (15 μg/ml) treatment. Approximately 30–40 μg of protein samples were loaded in each lane. No change in the level of expression of pro-caspase 3 protein or in its cleavage to caspase 3 was observed after treatment with oligonucleotides. Compared with G4126, G3139 dramatically down-regulated the expression of several S-phase genes, including c-myb and PARP. However, no PARP cleavage products were observed. Levels of cdk2, cdk4, c-myc, α-tubulin, and cyclin D1 expression also remained essentially unaltered after 3 days. Jonathan C. Lai et al. Mol Cancer Ther 2003;2:1031-1043 ©2003 by American Association for Cancer Research