Fig. 3. GABA content was increased in reactive astrocytes from 3D culture and hippocampal brain slice. (A) Immunostaining of neuron-astrocyte 3D co-culture.

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Fig. 3. GABA content was increased in reactive astrocytes from 3D culture and hippocampal brain slice. (A) Immunostaining of neuron-astrocyte 3D co-culture with anti-GFAP, anti-GABA antibodies and Hoechst. (B) Quantification of GABA immunoreactivity in control and adenovirus-infected astrocytes. *p<0.05, unpaired student's t-test. (C) Immunostaining of adenovirus-injected hippocampal brain slice with anti-GFAP, anti-GABA antibodies and Hoechst. (D) Quantification of GABA immunoreactivity in control and adenovirus-infected conditions. ***p<0.001, unpaired student's t-test. (E) Schematic for virus injection into DG hippocampus. DG, dentate gyrus; ML, molecular layer. Fig. 3. GABA content was increased in reactive astrocytes from 3D culture and hippocampal brain slice. (A) Immunostaining of neuron-astrocyte 3D co-culture with anti-GFAP, anti-GABA antibodies and Hoechst. (B) Quantification of GABA immunoreactivity in control and adenovirus-infected astrocytes. *p<0.05, unpaired student's t-test. (C) Immunostaining of… Exp Neurobiol. 2017 Jun;26(3):158-167. https://doi.org/10.5607/en.2017.26.3.158