Transfer of frozen–thawed blastocysts that underwent quarter laser-assisted hatching at the day 3 cleaving stage before freezing F.u-Tsai Kung, M.D.,

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Presentation transcript:

Transfer of frozen–thawed blastocysts that underwent quarter laser-assisted hatching at the day 3 cleaving stage before freezing F.u-Tsai Kung, M.D., Y.i-Chi Lin, B.Sc., Y.a-Jung Tseng, B.Sc., F.u-Jeng Huang, M.D., Meng-Ying Tsai, M.D., Shiuh-Young Chang, M.D. Fertility and Sterility Volume 79, Issue 4, Pages 893-899 (April 2003) DOI: 10.1016/S0015-0282(02)04846-X

FIGURE 1 Demonstration and localization of zona pellucida (ZP) breaching of embryos undergoing laser-assisted hatching under phase-contrast inverted microscopy (original magnification, ×400). (A), One quarter convex of the ZP of a cleaving embryo is thinned to 5 μm in thickness (arrowheads) with a central dimple, without penetrating the ooplasm (arrow). (B), The laser-breached ZP is identified and looks like a straight dissection (arrowheads) on the convex surface when the manipulated embryo is rotated. (C), The laser-hatched thinner convex ZP (arrowheads) is present in an expanded blastocyst. (D), The straight dissection on the ZP (arrowheads) is present in a thawed blastocyst examined immediately after thawing. ICM = inner cell mass; V = viable cells waiting for reexpansion; H = hyalinized dead cells with vacuoles. Kung. Transfer of frozen-thawed blastocysts. Fertil Steril 2003. Fertility and Sterility 2003 79, 893-899DOI: (10.1016/S0015-0282(02)04846-X)