FCM and TCR γ gene rearrangement analysis in LGL leukemia.

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FCM and TCR γ gene rearrangement analysis in LGL leukemia. FCM and TCR γ gene rearrangement analysis in LGL leukemia. (A) FCM analysis of a typical case of T CD3+ LGL leukemia showing CD3+/CD5dim/CD8+ (dark green). (B) FCM analysis of a case of NK CD3− LGL leukemia showing a CD3−/CD8−/CD16+/CD56+ phenotype (pink). KIR monotypic expression using CD161 and NkB1. (C) Clonality assessment: specific Vβ mAbs showing restricted expression of Vβ13.1 >90% (left); detection of clonal TCR γ gene recombination by gene scanning analysis, showing single peak in green (right). (D) Stat 3 mutation detection in a case of T-LGL leukemia using Sanger sequencing. FCM, flow cytometry; WT, wild-type. Thierry Lamy et al. Blood 2017;129:1082-1094 ©2017 by American Society of Hematology