IgE reactivity to α1 and α2 chains of bovine type 1 collagen in children with bovine gelatin allergy Masahiro Sakaguchi, PhDa, Hisae Hori, PhDc, Shunji.

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IgE reactivity to α1 and α2 chains of bovine type 1 collagen in children with bovine gelatin allergy Masahiro Sakaguchi, PhDa, Hisae Hori, PhDc, Shunji Hattori, PhDd, Shinkichi Irie, PhDd, Atsushi Imai, DVMf, Makoto Yanagida, PhDf, Hiroshi Miyazawa, PhDe, Masako Toda, PhDa, Sakae Inouye, MDb Journal of Allergy and Clinical Immunology Volume 104, Issue 3, Pages 695-699 (September 1999) DOI: 10.1016/S0091-6749(99)70344-1 Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 1 IgE reactivity to bovine gelatin and denatured type I collagen. Each child’s serum was diluted to approximately 1 Ua/mL as level of specific IgE to gelatin. Journal of Allergy and Clinical Immunology 1999 104, 695-699DOI: (10.1016/S0091-6749(99)70344-1) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 2 CM column chromatography of type I collagen. NaCl, Sodium chloride. Journal of Allergy and Clinical Immunology 1999 104, 695-699DOI: (10.1016/S0091-6749(99)70344-1) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 3 Analysis of SDS-PAGE (a) and immunoblotting (b). Lane 1, Denatured type I collagen; lane 2, α1 chain; lane 3 , α2 chain. Journal of Allergy and Clinical Immunology 1999 104, 695-699DOI: (10.1016/S0091-6749(99)70344-1) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 4 IgE reactivity to α1 and α2 chains. Each child’s serum was diluted to approximately 1 Ua/mL as level of specific IgE to gelatin. Journal of Allergy and Clinical Immunology 1999 104, 695-699DOI: (10.1016/S0091-6749(99)70344-1) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 5 Histamine release from mast cells passive sensitized with sera of bovine-sensitive children. Journal of Allergy and Clinical Immunology 1999 104, 695-699DOI: (10.1016/S0091-6749(99)70344-1) Copyright © 1999 Mosby, Inc. Terms and Conditions