Fig. 3 Classification of gene essentiality by transposon mutagenesis.

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Fig. 3 Classification of gene essentiality by transposon mutagenesis. Classification of gene essentiality by transposon mutagenesis. (A)Examples of the three gene classifications, based on Tn5 mutagenesis data. The region of syn1.0 from sequence coordinates 166,735 to 170,077 is shown. The gene MMSYN1_0128 (lime arrow) has many P0 Tn5 inserts (black triangles) and is an i-gene (quasi-essential). The next gene, MMSYN1_0129 (light blue arrow), has no inserts and is an e-gene (essential). The last gene, MMSYN1_0130 (gray arrow), has both P0 (black triangles) and P4 (magenta triangles) inserts and is an n-gene (nonessential). Intergenic regions are indicated by black lines. (B)The number of syn1.0 genes in each Tn5-mutagenesis classification group. The n- and in-genes are candidates for deletion in reduced genome designs. Clyde A. Hutchison III et al. Science 2016;351:aad6253 Copyright © 2016, American Association for the Advancement of Science