Genomic Sequence of Ufo1-1 Reveals a Novel CACTA Transposon Insertion in the Candidate Gene. Genomic Sequence of Ufo1-1 Reveals a Novel CACTA Transposon.

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Genomic Sequence of Ufo1-1 Reveals a Novel CACTA Transposon Insertion in the Candidate Gene. Genomic Sequence of Ufo1-1 Reveals a Novel CACTA Transposon Insertion in the Candidate Gene. (A) The PacBio genomic assembly of Ufo1-1 was aligned with the reference genome (B73 RefGenV4) to identify large presence/absence variations (PAVs, shown in red). Analysis of the candidate gene GRMZM2G053177 (gene model at bottom) shows the presence of a CACTA transposon (red triangle), in which the Ufo1-1 transcript begins. (B) Unique transcript of GRMZM2G053177 in Ufo1-1 plants. The first four amino acids (Met-Glu-Gln-Gln) encoded by the candidate gene in B73, and absent in Ufo1-1, are underlined. The amino acid sequence is shown in red below the nucleotide sequence. The rest of the transcripts following the ellipses (. . .) are identical. Kameron Wittmeyer et al. Plant Cell 2018;30:3006-3023 ©2019 by American Society of Plant Biologists