Possible Pathogenic Role of Th17 Cells for Atopic Dermatitis

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Presentation transcript:

Possible Pathogenic Role of Th17 Cells for Atopic Dermatitis Chizuko Koga, Kenji Kabashima, Noriko Shiraishi, Miwa Kobayashi, Yoshiki Tokura Journal of Investigative Dermatology Volume 128, Issue 11, Pages 2625-2630 (November 2008) DOI: 10.1038/jid.2008.111 Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Lactate dehydrogenase levels in different severity groups of AD patients. The AD patients were classified as acute (n=23), mild (n=7), moderate (n=5), and severe (n=11) by clinical findings, and serum levels of lactate dehydrogenase from AD patients were compared with that in healthy donors. Student's t-test was performed between the indicated groups and an asterisk indicates P< 0.05. Journal of Investigative Dermatology 2008 128, 2625-2630DOI: (10.1038/jid.2008.111) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Flow cytometric analysis of IL-17+ cells in peripheral blood lymphocytes. PBMCs isolated from a 30-year-old man with AD were stimulated with PMA and ionomycin for 8hours and Golgistop was added. Then, production of IL-17 by PBMCs was determined at the single-cell level by intracellular cytokine staining and flow cytometric analysis. The numbers in the upper and lower right quadrants represent the percentage of IL-17+ cells with or without expression of CD3+ or CD8+ cells in the total lymphocyte populations gated. Journal of Investigative Dermatology 2008 128, 2625-2630DOI: (10.1038/jid.2008.111) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Percentage of circulating IL-17+ T cells in relation to AD severity and IFN-γ+ T cells. AD patients were divided into three different severity groups (mild, n=7; moderate, n=5; and severe, n=11). (a) Healthy subjects (n=9), three groups of AD, psoriatic patients (n=16) were compared for percentage of IL-17+ and CD4+ or CD8+ T cells, and a significant correlation between healthy donors and severe AD patients was found (P=0.03). (b) IFN-γ+ T cells of all AD patients were also analyzed. A significant correlation between percentage of IL-17+ CD4+ T cells and IFN-γ+ T cells was found (P=0.0009). In addition, the correlation (P-value) between the percentage of IL-17+ CD4+ T cells and IFN-γ+ T cells among acute, moderate, and severe AD patients was depicted. Journal of Investigative Dermatology 2008 128, 2625-2630DOI: (10.1038/jid.2008.111) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Immunohistochemistry of IL-17+ cells in skin lesions of AD. (a) A skin specimen from an AD patient was immunohistochemically stained for IL-17. Left, low-magnification image of acute lesion; top right, high-magnification image of acute lesion; and bottom right, high-magnification image of chronic lesion. Bar=30μm. (b) The number of IL-17+ cells was enumerated in acute and chronic lesions from mild, moderate, and severe cases of AD. Columns show mean±SD. Student's t-test was performed between the indicated groups and an the asterisk indicates P<0.05. Journal of Investigative Dermatology 2008 128, 2625-2630DOI: (10.1038/jid.2008.111) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Effects of IL-17 and/or IL-22 on keratinocyte production of cytokines/chemokines and VEGF. NHEKs were cultured with or without IL-17 and/or IL-22 at the indicated doses. Three-day culture supernatants were subjected to analysis with the cytometric beads array system or ELISA. Columns show mean±SD of data using triplicated wells. The asterisk indicates statistically significant differences compared with the untreated group (P<0.05, unpaired two-tailed t-test). Data are representative of those from three independent experiments. Journal of Investigative Dermatology 2008 128, 2625-2630DOI: (10.1038/jid.2008.111) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions