B7-H1–induced reverse signals in myeloma cells.

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B7-H1–induced reverse signals in myeloma cells. B7-H1–induced reverse signals in myeloma cells. A, mRNA expression of BCL2, FASLG, and FADD was quantified using real-time PCR in PD-1-Fc- or control Ig bead–treated MOSTI-1 cells. B, Western blotting analysis of PI3K, p-AKT, AKT, BCL2, caspase-3, cleaved caspase-3, and β-actin in melphalan (MEL)- or bortezomib (BOR)-treated MOSTI-1 cells after incubation with PD-1-Fc or control Ig beads. C, relative intensity of the p-Akt protein was calculated based on the signals of p-Akt and Akt. D, relative intensity of the BCL2 and cleaved caspase-3 to β-actin was calculated based on the signals of these proteins, and the data are expressed as mean ± SD of duplicate experiments. Akt and p-Akt, 60 kDa; PI3K, 85 kDa; BCL2, 28 kDa; caspase-3, 35 kDa; cleaved caspase-3, 17 kDa; β-actin, 42 kDa. *, P < 0.05; **, P < 0.01. Mariko Ishibashi et al. Cancer Immunol Res 2016;4:779-788 ©2016 by American Association for Cancer Research