Involvement of Crm1 in the hemin‐induced nuclear exclusion of Bach1

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Heme regulates gene expression by triggering Crm1‐dependent nuclear export of Bach1 Involvement of Crm1 in the hemin‐induced nuclear exclusion of Bach1. (A, B) Localization of GFP‐GST‐B1(417–587) in 293T cells (A) or Bach1 in GM02063 cells (B) in the presence of Crm1 or Crm1K1 was examined. Cells were treated with indicated reagents. (C) Glutathione beads (lane 4) or glutathione beads with GST–Bach1(417–645) (lane 5) were incubated with liver cell extracts and bound proteins were analyzed by immunoblotting analysis using anti‐Crm1 antibodies. Fractions of input extracts were also examined (5.7, 1.9, or 0.6 μg in lanes 1–3, respectively). IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 23, Issue: 13, Pages: 2544-2553, First published: 03 June 2004, DOI: (10.1038/sj.emboj.7600248)