Gregor Cevc, Dieter Gebauer Biophysical Journal

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Presentation transcript:

Hydration-Driven Transport of Deformable Lipid Vesicles through Fine Pores and the Skin Barrier Gregor Cevc, Dieter Gebauer Biophysical Journal Volume 84, Issue 2, Pages 1010-1024 (February 2003) DOI: 10.1016/S0006-3495(03)74917-0 Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 1 Water adsorption isotherms of phosphatidylcholine (multi)bilayers and of highly flexible mixed lipid membranes consisting of SPC and sodium cholate at room temperature (A). Effective hydration decay length (B) and surface hydration potential (C) as a function of water activity in the system. Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 2 Temporal dependence of vesicle transport across a nano-porous membrane (rpore=30nm) as a function of transbarrier water activity gradient (occlusion versus nonocclusion). Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 3 Effect of transbarrier water humidity or activity gradient on transport of highly deformable vesicles across a barrier with narrow pores (rv/rpore=2.7). Upper panel gives the flux as a function of time; lower panel provides the corresponding barrier penetrability values (flux derivative, bullets) and water adsorption isotherm for a comparable lipid membrane (curve). Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 4 Effect of changing excess water volume on the flux of ultraadaptable vesicles across a barrier with small pores (rv/rpore=2.7) as a function of time. Dashed lines give 95% confidence limit. Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 5 Lag time for penetration of ultraadaptable mixed lipid vesicles across a nano-porous barrier as a function of water activity gradient (upper panel; derived from Fig. 3) or of excess water volume in donor compartment (lower panel). Inset illustrates the insensitivity of flux for the latter data set and is derived from Fig. 4. Lines are results of linear fits to the data; dashed lines give 95% confidence limits. Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 6 Control of transbarrier flux by changing vesicle shape adaptability by changing the composition of mixed lipid bilayers, under conditions of constant relative penetrant size (rv/rpore=3) and transbarrier water activity gradient (RH=30%). Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 7 Effect of vesicle size and adaptability, changed by varying lipid bilayer composition, on the penetrability of a barrier to large vesicles (upper panel) or on lag time for transbarrier transport (lower panel), measured at 30% relative humidity. Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 8 Effect of vesicle adaptability, varied by changing bilayer composition, on the normalized transport of relatively large (rv/rpore=3; upper panel) and small (rv/rpore=1.8; lower panel) vesicles across a barrier with 30-nm pores, measured at 30% relative humidity. (Values in horizontal range are not significantly different from the lower detection limit.) Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 9 Vesicle-mediated transport of fluorescent label DPH across intact, excised skin as a function of time, driven by transcutaneous water activity gradient. Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 10 Transport of various lipid aggregates across nonoccluded human skin. Biophysical Journal 2003 84, 1010-1024DOI: (10.1016/S0006-3495(03)74917-0) Copyright © 2003 The Biophysical Society Terms and Conditions