Workflow summary of printed microarrays.

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Presentation transcript:

Workflow summary of printed microarrays. Workflow summary of printed microarrays. Probes are PCR amplified (or oligonucleotides are synthesized) and subsequently spotted onto a glass slide. In this example, two samples to be compared undergo RNA extraction, cDNA production, and differential fluorescent labeling. Hybridization of labeled target nucleic acids to the probe array allows fluorescent scanning to provide data for analysis. (Adapted from reference 51 [Fig. 1A, © Springer-Verlag 2006] with kind permission from Springer Science and Business Media.)‏ Melissa B. Miller, and Yi-Wei Tang Clin. Microbiol. Rev. 2009; doi:10.1128/CMR.00019-09