HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC‐to‐EpiSC/hESC transition Expression of nuclear‐encoded mitochondrial.

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HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC‐to‐EpiSC/hESC transition Expression of nuclear‐encoded mitochondrial IV COX genes is lower in EpiSC compared with ESC, resulting in lower complex IV activity in EpiSC. (A) EpiSC have lower mitochondrial membrane potential than ESC and MEFs measured by TMRM staining. (B) Heatmap of microarray expression data of mitochondrial complex IV COX gene cluster is shown, where EpiSC clearly demonstrate lower expression in these genes than ESC. (C) Validation of Cox8c, Cox6b2, Cox7a1 and NqoI by quantitative PCR assays. (D) Complex IV isolated from EpiSC or hESC (H1 and H7 lines) shows lower activity in vitro than that of ESC; the activity of Complex IV isolated from MEFs is shown as comparison. (E) Potential regulators involved in mitochondria respiration, SCO2, PGC‐1β and Esrrb, express at lower levels in EpiSC. Results were summarized from three independent biological samples, and the error bars represented the standard error of the mean. *Indicates P<0.05, ** indicates P<0.01. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 31, Issue: 9, Pages: 2103-2116, First published: 23 March 2012, DOI: (10.1038/emboj.2012.71)