Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system.

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Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. The bundle of the flat curves includes 33 wild-type measles virus specimens comprising the following genotypes: B2, B3, C1, C2, D2, D3, D4, D5, D6, D7, D8, D9, D10, G1, G2, E, H1, and H2. The amplification curves are from MeV vaccine RNA from 102 to 105 copy numbers, assessed in duplicate. The QuantiTect Probe RT-PCR kit was used for these reactions. Felicia Roy et al. J. Clin. Microbiol. 2017; doi:10.1128/JCM.01879-16