Figure 5. Hematopoietic cell specific Atg5 deficiency results in aberrant proliferation and mitochondrial dysfunction in hematopoietic cells. (A) Apoptosis and cell proliferation assays of LSKs from control and Vav_Atg5−/− mice were performed using Annexin V, BrdU, and Ki67 staining. For the BrdU assay, proliferation of LSK cells was analyzed 24 h after BrdU injection (n=4–5). (B) Approximately 2×106 of total bone marrow cells from control and Vav_Atg5−/− mice were injected intravenously into lethally irradiated CD45.1+ mice. After 8 weeks, percentage of reconstitution of transplanted bone marrow in peripheral blood was confirmed by flow cytometry (n=3). (C) Mitochondrial functions in bone marrow LSK cells from control and Vav_Atg5−/− mice were assessed using MitoTracker Green, MitoTracker Red, and MitoSox (n=4–5). Data are representative of 3 independent experiments and presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 as calculated by Student's t-test. Figure 5. Hematopoietic cell specific Atg5 deficiency results in aberrant proliferation and mitochondrial dysfunction in hematopoietic cells. (A) Apoptosis and cell proliferation assays of LSKs from control and Vav_Atg5−/− mice were performed using Annexin V, BrdU, and Ki67 staining. For the BrdU assay, proliferation of LSK cells was analyzed 24 h after… Immune Netw. 2019 Apr;19(2):e12. https://doi.org/10.4110/in.2019.19.e12