Effects of follicular depletion induced by VCD and chronic 17β-estradiol (E) administration on the relative mRNA expression of PR (left panel) and ERβ.

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Effects of follicular depletion induced by VCD and chronic 17β-estradiol (E) administration on the relative mRNA expression of PR (left panel) and ERβ (right panel) in the DRN. Female rats at 28 d of age were subcutaneously injected with corn oil (O) or VCD... Effects of follicular depletion induced by VCD and chronic 17β-estradiol (E) administration on the relative mRNA expression of PR (left panel) and ERβ (right panel) in the DRN. Female rats at 28 d of age were subcutaneously injected with corn oil (O) or VCD for 15 consecutive days. Fifty-five days after the first injection of VCD/oil, rats received a subcutaneous capsule that contained corn oil (groups O + O and VCD + O) or 17β-estradiol (VCD + E). Rats were decapitated during the diestrous phase (O + O and VCD + O groups) or 21 d after the onset of E therapy (VCD + E), from 75 to 85 d after the onset of VCD/oil administration, between 9:00 and 11:00 A.M. (n = 6-9). Data are presented as the mean ± SEM. Significance was accepted at p < 0.05. Nayara Pestana-Oliveira et al. eNeuro 2018;5:ENEURO.0247-17.2017 ©2018 by Society for Neuroscience