Performance and potency comparisons of qHTS runs.

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Performance and potency comparisons of qHTS runs. Performance and potency comparisons of qHTS runs. A and B, plate performance measures of signal to background ratio (S/B) and Z′-factor based on positive (EC100 podophyllotoxin) and negative (DMSO alone) control wells for each plate of two qHTS runs (•, ○) of SW480 (A) and MCF10A (B) cells. The Z′-factor was calculated as previously described (8). C and D, the potencies of active and inconclusive compounds were reproducible between qHTS runs for each cell line. Plots of log EC50 values were fitted by linear regression and showed correlation for SW480 (r2 = 0.9, n = 68) and MCF10A (r2 = 0.81, n = 195) cells. Wenge Zhu et al. Mol Cancer Res 2011;9:294-310 ©2011 by American Association for Cancer Research