Potentiation of proton-gated current in CHO cells expressing ASICs

Slides:

Advertisements

Similar presentations

Fig. 1 (A) Average current voltage relations of peak INa in Con (n=10), nAF (n=7) and cAF (n=9) cells using protocol shown on left. (B) Average.

Advertisements

Figure 1 A high level of Ca2+ influx achieved by prolonging the depolarizing pulse abolishes the inhibitory effect of PGE1 on exocytosis. A high level.

Rate of voltage clamp-induced depolarization of DVR endothelial layer with di-8-ANEPPS. Rate of voltage clamp-induced depolarization of DVR endothelial.

Fig. 1 Inhibition of hERG channels by bisindolylmaleimide I

Requirement for a β subunit for the modulation of whole-cell CaV2

Functional Modularity of the β-Subunit of Voltage-Gated Ca2+ Channels

Volume 95, Issue 5, Pages e11 (August 2017)

Endocannabinoids Control the Induction of Cerebellar LTD

Differential Modulation of Cardiac Ca2+ Channel Gating by β-Subunits

Iman M Shammat, Sharona E Gordon Neuron

Volume 81, Issue 1, Pages (January 2014)

Down-regulation of L-type calcium channels in inflamed circular smooth muscle cells of the canine colon Xiaorong Liu, Nancy J. Rusch, Joerg Striessnig,

Volume 110, Issue 11, Pages (June 2016)

Coincident Pre- and Postsynaptic Activity Modifies GABAergic Synapses by Postsynaptic Changes in Cl− Transporter Activity Melanie A Woodin, Karunesh.

Unitary Conductance Variation in Kir2

Pacemaking by HCN Channels Requires Interaction with Phosphoinositides

A. Dinse, K.J. Fo¨hr, M. Georgieff, C. Beyer, A. Bulling, H.U. Weigt

Jian Liu, Steven A. Siegelbaum Neuron

David C. Immke, Edwin W. McCleskey Neuron

Volume 11, Issue 1, Pages (January 2014)

Volume 106, Issue 12, Pages (June 2014)

Jason Jacoby, Yongling Zhu, Steven H. DeVries, Gregory W. Schwartz

Volume 14, Issue 11, Pages (November 2017)

Fast Removal of Synaptic Glutamate by Postsynaptic Transporters

Modulation of the Gating of Unitary Cardiac L-Type Ca2+ Channels by Conditioning Voltage and Divalent Ions Ira R. Josephson, Antonio Guia, Edward G.

SK2 Channel Modulation Contributes to Compartment-Specific Dendritic Plasticity in Cerebellar Purkinje Cells Gen Ohtsuki, Claire Piochon, John P. Adelman,

Figure 2 PGE1 inhibits endocytosis.

Volume 96, Issue 10, Pages (May 2009)

Amanda H. Lewis, Alisa F. Cui, Malcolm F. McDonald, Jörg Grandl

Yael Stern-Bach, Sebastian Russo, Menahem Neuman, Christian Rosenmund

Kinetic and Energetic Analysis of Thermally Activated TRPV1 Channels

Volume 23, Issue 3, Pages (July 1999)

Distribution and functionality of Arch-EGFP pumps in the peripheral sensory pathways of Nav1.8-Arch+ mice. Distribution and functionality of Arch-EGFP.

Tonic Inhibition of TRPV3 by Mg2+ in Mouse Epidermal Keratinocytes

Whole-cell patch-clamp analysis of NAc cells.

The fluorescent receptor chains exhibit their characteristic fluorescent signatures: images and spectra of human IFN-γR2/GFP and IFN-γR2/EBFP transfected.

Synaptic transmission and plasticity is disturbed in 2-week-old but not in adult SynDIG1 β-gal mutant mice. Synaptic transmission and plasticity is disturbed.

Katie C. Bittner, Dorothy A. Hanck Biophysical Journal

KCNKØ: Single, Cloned Potassium Leak Channels Are Multi-Ion Pores

Excitability of the Soma in Central Nervous System Neurons

Gabe J. Murphy, Fred Rieke Neuron

Light illumination excites Lamina I SDH neurons after PNI

Inhibition of αβ Epithelial Sodium Channels by External Protons Indicates That the Second Hydrophobic Domain Contains Structural Elements for Closing.

Volume 26, Issue 1, Pages (April 2000)

Current Injection Provokes Rapid Expansion of the Guard Cell Cytosolic Volume and Triggers Ca2+ Signals Lena J. Voss, Rainer Hedrich, M. Rob G. Roelfsema

Jeffrey S Diamond, Dwight E Bergles, Craig E Jahr Neuron

Regulating the Conducting States of a Mammalian Serotonin Transporter

Pitx3-GFP+ cells exhibit functional neuron-like electrophysiological properties. Pitx3-GFP+ cells exhibit functional neuron-like electrophysiological properties.

Selective modulation of AMPAR-mediated transmission in 4E-BP2−/− mice.

Prominent ADP following action potentials at MFBs in mouse hippocampal slices. Prominent ADP following action potentials at MFBs in mouse hippocampal slices.

Neurons in the developing spinal cord express inwardly rectifying K+ channels other than Kir4.1.A, Voltage protocol. Neurons in the developing spinal cord.

CIE does not alter D2/D4 receptor expression in the mPFC.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

Ih activation is required for sigh but not for eupnea generation.

NRG1 depolarizes action potential threshold.

Application of GABA to nucleated patches from dentate granule cells induces SR95531-sensitive channel openings. Application of GABA to nucleated patches.

Spontaneous EPSC and IPSC dynamics in 3 mm and 1 mm Ca2+.

Volume 95, Issue 5, Pages e4 (August 2017)

Effects of an AMPA receptor antagonist on numbers of pCofilin-positive spines. Effects of an AMPA receptor antagonist on numbers of pCofilin-positive spines.

Use Dependence of Heat Sensitivity of Vanilloid Receptor TRPV2

Knock-out of NSG2 decreases mEPSC frequency.

PSCs elicited by electrical stimulation of the subplate.

Glutamate responses are enhanced by coapplication of IMP, consistent with umami taste stimulation. Glutamate responses are enhanced by coapplication of.

Thy1S promoter drives sparse expression of hrGFP in marmoset cortex.

Volume 37, Issue 1, Pages (January 2003)

Target-specific DSI at TRN synapses.

Depression of NMDA receptor-mediated EPSCs

A, Representative examples of Ca currents elicited by repetitive 3 Hz depolarizing voltage steps to 0 mV from a holding potential of −40 mV from a CA1.

A, Representative voltage-clamp recordings from a CRF1+ neuron during superfusion of GBZ (100 μm), followed by PTX (100 μm, top trace) and during superfusion.

Leptin-mediated electrophysiological activation of POMC neurons.

Presentation transcript:

Potentiation of proton-gated current in CHO cells expressing ASICs Potentiation of proton-gated current in CHO cells expressing ASICs. Whole-cell patch clamp, holding potential −60 mV. Potentiation of proton-gated current in CHO cells expressing ASICs. Whole-cell patch clamp, holding potential −60 mV. A, ASIC1a: application of pH 6.9 solution (open bar) before (left trace), during (middle trace), and after (right trace) the application of 100 μm SNAP (black bar). B, Corresponding bar graph in which the current is normalized to the control (100%, left column; n = 9; **p < 0.01). C, Current in ASIC1a transfected CHO cells in response to low pH in the absence (top) and in the presence (black box) of 100 μm SNAP. D, Dependence of peak current on pH in absence (open squares) and presence (black squares) of SNAP (n = 7). E–G, Enhancement of activity of ASIC1b, ASIC2a, and ASIC3 by SNAP (5 s pulses of pH 6.0, 6.0, and 6.9 respectively). *p < 0.05; **p < 0.01. Hervé Cadiou et al. J. Neurosci. 2007;27:13251-13260 ©2007 by Society for Neuroscience