Interaction of aggregates with CAF network structures.

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Interaction of aggregates with CAF network structures. Interaction of aggregates with CAF network structures. A, bright field micrographs of CAF structures alone (left panel), aggregates of 344SQ cells alone (middle panel), or 344SQ aggregates seeded on CAF structures (right panel). Bar size, 200 μm. B, bright field micrographs of 344SQ aggregates seeded on CAF structures in the presence of latrunculin A or blebbistatin. Bar size, 200 μm. C, bright field (top left panel) and fluorescence micrographs of a RFP-tagged tumor aggregate attached to CAF structures (green) imaged 2 days after seeding. Arrow points to region of aggregate that is beginning to dissociate and migrate along CAF networks. F-actin (green). Bar size, 20 μm. D, immunofluorescence microscopic images of 2 RFP-expressing tumor aggregates (red) that have disorganized, migrated toward each other, and coalesced (arrow) on CAFs. CAFs pretreated with cell tracker green. Photographed 2 days after seeding. Inset is Z-stack image of bracketed area. Bar size, 100 μm. Daniela Pankova et al. Mol Cancer Res 2016;14:287-295 ©2016 by American Association for Cancer Research