Activity of SW480 qHTS runs and podophyllotoxin titration–response profiles of from library and control samples. Activity of SW480 qHTS runs and podophyllotoxin titration–response profiles of from library and control samples. A, activity heat maps of plates incubated with increasing concentrations of the LOPAC1280 compounds. The percent activity of each well was calculated relative to podophyllotoxin (defined as 100% activity) and DMSO (defined as 0% activity). The results are depicted here as a gradient of color where white, blue, and red indicate no, increasing, and decreasing activity, respectively, relative to DMSO-treated control wells. These data were converted into dose–response curves. Podophyllotoxin dose–response curves from the qHTS runs (B and C) in which each of 7 plates containing the concentration indicated in (A) are shown for SW480 (B) and MCF10A (C) cells. Intraplate control titrations of 16 different concentrations of podophyllotoxin (D and E) are shown for SW480 (D) and MCF10A (E) cells. Each panel contains a dose–response curve from two runs (○ dashed line, • solid line). Wenge Zhu et al. Mol Cancer Res 2011;9:294-310 ©2011 by American Association for Cancer Research