Fig. 1 Data collection at a 40° tilt angle enabled reconstruction of a cryo-EM map of the NRTN-GFRα2-RET complex at a 5.7-Å resolution. Data collection.

Slides:

Advertisements

Similar presentations

Fig. 2 Chiral and achiral structures of formic acid.

Advertisements

Fig. 2 Transport properties of a BP transistor at low temperature.

Fig. 4 Ballistic simulation of BP FETs.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 2 CFD results. CFD results. Results of CFD simulations in horizontal (left column) and vertical (right column) cross-sections. All models oriented.

Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 1 Map of water stress and shale plays.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 3 Saturation velocity of BP FETs.

Fig. 4 Morphogenesis in the Ch-CNC host droplets and NP assemblies.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

Fig. 6 Comparison of properties of water models.

Fig. 2 Average number of fake news shares (and 95% CIs) using the list of domains derived from (7). Average number of fake news shares (and 95% CIs) using.

Fig. 4 Latency of CsLoc during testing.

Fig. 2 Cryo-EM structure of Elp123 bound to tRNAAlaUGC.

Fig. 3 Scan rate effects on the layer edge current.

Fig. 2 Stratigraphic profile of the Area 15 excavation block showing the diagnostic cultural materials and components alongside the stratigraphic sequence.

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Study area and field site setting.

Fig. 1 Cryo-EM structure of yeast Elp123 showing its active site.

Fig. 1 Reference-fixing experiment, setup.

Fig. 1 Schematic illustration of the preparation and potential application of FMSMs. Schematic illustration of the preparation and potential application.

Fig. 1 Parameterization and temporal distribution of carbon isotopic events in the database. Parameterization and temporal distribution of carbon isotopic.

Fig. 1 Distribution of total and fake news shares.

Fig. 4 Vent forecasts. Vent forecasts. Top: Stationary stress forecast. (A) Thin red lines are forecasted magma trajectories, and red stairs indicate the.

Fig. 5 Analysis of VLPs formed from deletion CP mutants.

Fig. 3 Photon number statistics resulting from Fock state |l, S − l〉 interference. Photon number statistics resulting from Fock state |l, S − l〉 interference.

Fig. 2 2D QWs of different propagation lengths.

Fig. 5 Molecular dynamics simulations of Rac1.

Electronic structure of the oligomer (n = 8) at the UB3LYP/6-31G

Fig. 4 DFT ωB97x/def2-TZVPP atomic charges on the sulfur atom of substituted thioaldehyde and AIMNet prediction with a different number of iterative passes.

Fig. 5 In-plane angle dependence of SOT efficiency (θDL,m) and resonance condition (Hres). In-plane angle dependence of SOT efficiency (θDL,m) and resonance.

Fig. 2 Cardiolipin binding sites in complex I.

Fig. 1 Histograms of the number of first messages received by men and women in each of our four cities. Histograms of the number of first messages received.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 1 Average contribution (million metric tons) of seafood-producing sectors, 2009–2014. Average contribution (million metric tons) of seafood-producing.

Fig. 4 Two-color photoinitiation and photoinhibition enable controllable, far-surface patterning of complex 3D structures. Two-color photoinitiation and.

Fig. 4 Praying Prophet by Lorenzo Monaco: Mapping lake pigments and associated substrate. Praying Prophet by Lorenzo Monaco: Mapping lake pigments and.

Fig. 1 Structure of the α-SNAP–SNARE subcomplex.

Fig. 2 Characterization of ZnxCo1−xO NRs.

Specificity profiling, activity in human plasma, and x-ray structure

Fig. 2 Schematic drawings of Göbekli Tepe skulls.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Schematic of the proposed brain-controlled assistive hearing device

Fig. 5 Skull 1: Tentative reconstruction.

Fig. 4 BS-SEM images, ternary diagrams, and phase maps for the text and reverse sides of the TS. BS-SEM images, ternary diagrams, and phase maps for the.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 Map of δ18OVSMOW in groundwaters of the British Isles (left) and Strontium (87Sr/86Sr) biosphere map of Great Britain (right). Map of δ18OVSMOW.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 2 Simulations of possible doping positions and band structures.

Fig. 3 Comparisons of NDVI trends over the globally vegetated areas from 1982 to Comparisons of NDVI trends over the globally vegetated areas from.

Fig. 1 Schematic depiction of a paradigm for rapid and guided discovery of materials through iterative combination of ML with HiTp experimentation. Schematic.

Fig. 3 Electrochemical performances of symmetric cells using control Li and composite Li electrodes. Electrochemical performances of symmetric cells using.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 3 Structural variation under various dc voltages applied on the same one single crystal of FJU-23-H2O along the c axis. Structural variation under.

Fig. 4 Single-particle contact angle measurements.

Fig. 4 Analysis of complex formation and signaling capacity of wild-type and mutant proteins. Analysis of complex formation and signaling capacity of wild-type.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 4 Behavior of resistance peak near density nm = 5.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Fig. 4 Scaling laws distinguish biochemical networks from random networks across levels of organization. Scaling laws distinguish biochemical networks.

Fig. 4 Analysis of charge storage mechanism in ZnxCo1−xO NRs.

Fig. 2 Daily TNC pickups and drop-offs for an average Wednesday in fall 2016 (1). Daily TNC pickups and drop-offs for an average Wednesday in fall 2016.

Fig. 2 Speaker-independent speech separation with ODAN.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 1 Crystal structure of the MMOH-MMOD complex.

Fig. 4 Orthosteric ligand-binding pocket in CysLT1R.

Fig. 1 Completely derived from natural wood, nanowood with hierarchically aligned cellulose nanofibrils can be used as an anisotropic super thermal insulator.

Presentation transcript:

Fig. 1 Data collection at a 40° tilt angle enabled reconstruction of a cryo-EM map of the NRTN-GFRα2-RET complex at a 5.7-Å resolution. Data collection at a 40° tilt angle enabled reconstruction of a cryo-EM map of the NRTN-GFRα2-RET complex at a 5.7-Å resolution. (A) Comparison of two-dimensional (2D) class averages obtained from data collected at 0° or 40° tilt angles. 2D classes from the 0° data collection showed severe orientation bias of the molecule, exclusively presenting the view from the top revealing a figure 8 shape. 2D classes from data collected at the 40° tilt angle show a broader distribution of views. (B) Cryo-EM map reconstruction at a 5.7-Å resolution and atomic model derived from fitting in the cryo-EM map. Density around the NRTN dimer (or cartoon representation) is colored orange, GFRα2 is in blue, and RETCLD1-4 is in pink and magenta. Density for the unmodeled RETCRD is depicted in gray. Most of the density could be modeled, and only RETCRD could not be traced (gray, center). The side view of the complex suggests the position of the plasma membrane (right). (C) Schematic close-up view of the calcium-binding site between RETCLD2 and RETCLD3 (left), GFRα2 domains 1–3 showing the electron density (center), and the NRTN-GFRα2 interface mediated by the NRTN finger domain (right). Janna M. Bigalke et al. Sci Adv 2019;5:eaau4202 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).