β-Catenin/Tcf7l2 complex recruits Zic5 to regulate gene expression

Slides:

Advertisements

Similar presentations

Fig. 5 Correlation of RNA expression and protein abundance.

Advertisements

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 3 Oil, gas, and FP water variations with time.

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 1 Map of water stress and shale plays.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

Fig. 6 Comparison of properties of water models.

Fig. 1 Mean and median RCR (Relative Citation Ratio) of Roadmap Epigenomics Program research articles for each year. Mean and median RCR (Relative Citation.

Fig. 2 Reference-fixing experiment, results.

Fig. 5 Conservation of m6A in mammals.

Fig. 3 Scan rate effects on the layer edge current.

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 5 EGLN2-mediated HIF1A down-regulation promotes ferroptosis.

Fig. 2 Contribution of SQSTM1 to the autophagic degradation of ARNTL.

Fig. 4 Loss of Zic5 derepresses GLUT1/SLC2A1 gene expression.

Fig. 1 Distribution of total and fake news shares.

Fig. 1 Genes up-regulated in Zic5 KO cells correlate with the increased glycolytic state. Genes up-regulated in Zic5 KO cells correlate with the increased.

Fig. 2 2D QWs of different propagation lengths.

Fig. 6 Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent manner in HCT116 colon cancer cells. Zic5 regulates glucose metabolism in a β-catenin/Tcf7l2–dependent.

Fig. 5 Molecular dynamics simulations of Rac1.

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 4 Horizontal structures of the four types of MJO.

Fig. 1 Histograms of the number of first messages received by men and women in each of our four cities. Histograms of the number of first messages received.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

Fig. 2 Regulation of CD73 and CD39 cell membrane expressions and extracellular adenosine levels by ERs in osteoprogenitor cells. Regulation of CD73 and.

Fig. 1 Average contribution (million metric tons) of seafood-producing sectors, 2009–2014. Average contribution (million metric tons) of seafood-producing.

Fig. 3 Regulation of CD73 and CD39 cell membrane expression and extracellular adenosine levels by ERs in osteoclasts. Regulation of CD73 and CD39 cell.

Fig. 4 The mechanical performances of thermally stable click-ionogels.

Fig. 4 Visualization of complex loop motions by 1-μs MD trajectories.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

Fig. 2 Schematic drawings of Göbekli Tepe skulls.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 4 Projected [CO2]-induced deficits in protein and minerals (Fe and Zn) and cumulative changes in vitamin B and cumulative changes in vitamin E derived.

Fig. 2 Viperin promotes methionine oxidation of KSHV helicase.

Fig. 4 Adenosine A2BR signaling promote osteogenic and inhibit osteoclast differentiation in vitro. Adenosine A2BR signaling promote osteogenic and inhibit.

Fig. 1 Global occurrences of hydraulic fracturing–induced seismicity and potential models. Global occurrences of hydraulic fracturing–induced seismicity.

Fig. 1 Gradients of species richness and predicted turnover through extinction and redistribution. Gradients of species richness and predicted turnover.

Analysis of A-to-I RNA edits found in transcriptome-wide RNA-seq

Fig. 5 Global patterns in total fisheries catches from over more than 50 years as seen in three example stanzas. Global patterns in total fisheries catches.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Fig. 3 DUB proteins act at different levels of type I IFN pathways.

Fig. 1 Global distribution of surface DIP.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 3 Effects of ASO and eGLP1-ASO conjugates on gene expression and protein levels in vitro in cell lines and primary mouse islet cells. Effects of ASO.

Fig. 3 Comparisons of NDVI trends over the globally vegetated areas from 1982 to Comparisons of NDVI trends over the globally vegetated areas from.

Fig. 1 Schematic depiction of a paradigm for rapid and guided discovery of materials through iterative combination of ML with HiTp experimentation. Schematic.

Fig. 4 Calculated RIXS data of BCIO.

Fig. 4 Mapping of abundance of the most dominant bacterial and archaeal phyla across France. Mapping of abundance of the most dominant bacterial and archaeal.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 4 Single-particle contact angle measurements.

Fig. 5 Foxp3 promotes functional recruitment of Bcl11b in Treg cells.

Fig. 4 Changes in amount of atmospheric aerosol and of solar energy at Earth’s surface after nuclear exchange. Changes in amount of atmospheric aerosol.

Fig. 3 Supraballs and films assembled from binary 219/217nm SPs/SMPs.

Fig. 2 Supraballs and films from binary SPs.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 4 Behavior of resistance peak near density nm = 5.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Fig. 5 Cytosolic delivery of toxic proteins.

Fig. 1 B2 RNA directly inhibits transcription.

Fig. 6 Methionine oxidation catalyzed by viperin increases the stability and function of RNA helicase RIG-I. Methionine oxidation catalyzed by viperin.

Fig. 2 Daily TNC pickups and drop-offs for an average Wednesday in fall 2016 (1). Daily TNC pickups and drop-offs for an average Wednesday in fall 2016.

Fig. 4 Effects of individual picosecond and microsecond pulses.

Fig. 2 Combined transcriptome profiling, ChIP-seq, and ATAC-seq analysis identifies 14 highly plausible direct targets of Runx. Combined transcriptome.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 5 Enhancers and super-enhancers identified in various brain cell populations. Enhancers and super-enhancers identified in various brain cell populations.

Presentation transcript:

Fig. 5 β-Catenin/Tcf7l2 complex recruits Zic5 to regulate gene expression. β-Catenin/Tcf7l2 complex recruits Zic5 to regulate gene expression. (A) MudPIT analysis of the purification of 3×Flag-Zic5 and 3×Flag-Zic2 in HCT116 cells from nuclear extracts. (B and C) Validation of Tcf7l2 and β-catenin co-immunoprecipitation with Zic5 or Zic2 by Western blot. VT, vector control. (D) Tcf7l2 but not β-catenin directly binds to Zic5 and Zic2 in the in vitro binding assay. IB, immunoblotting. (E) C terminus of Zic5 is required to be directly associated with the β-catenin/Tcf7l2 complex. (F and G) CTNNB1 or TCF7L2 knockdown in HCT116 cells by distinct short hairpin RNAs (shRNAs). The protein levels of β-catenin, Tcf7l2, Zic2, and Zic5 were examined by Western Blot. (H) Average plot and (I) heat maps showing the global binding of Zic5 in shControl cells and shCTNNB1 or shTCF7L2 cells from −1 to +1 kb centered on Zic5 peaks in shControl cells. (J) Boxplot depicts the normalized reads coverage for Zic5 peaks in shControl cells and shCTNNB1 or shTCF7L2 cells. The P values were calculated with the Wilcoxon signed-rank test. (K and L) Track examples showing the Zic5 binding at the GLUT1 gene promoter region with Tcf7l2 or β-catenin knockdown. IgG, immunoglobulin G. Zibo Zhao et al. Sci Adv 2019;5:eaax0698 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).