Registry of Parts: http://parts.mit.edu.

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Presentation transcript:

Registry of Parts: http://parts.mit.edu

Types of Parts: http://parts.mit.edu

Registry of Parts: http://parts.mit.edu

Registry of Parts: http://parts.mit.edu

BioBrick Parts Assembly Strategy XbaI Spe1 EcoR1 PstI XbaI SpeI Mixed Site A CTAGA TGATC T

BioBricks Strategy: To add Downstream Parts BioBrick Vector BioBrick Part EcoR1 XbaI PartA Spe1 PstI EcoR1 XbaI PartB Spe1 PstI To add to end Cut with Spe1 and Pst1 To add to end Cut with Xba1 and Pst1 EcoR1 XbaI PartA Spe1 PstI XbaI PartB Spe1 PstI Ligate (Xba1 and Spe1 Have compatible Overhangs) EcoR1 XbaI PartA Mixed PartB Spe1 PstI Site A CTAGA TGATC T

BioBricks Strategy: To add Upstream Parts BioBrick Vector BioBrick Part EcoR1 XbaI PartA Spe1 PstI EcoR1 XbaI PartB Spe1 PstI Cut with EcoR1 and XbaI Cut with EcoR1 and Spe1 EcoR1 XbaI PartA Spe1 PstI EcoR1 XbaI PartB Spe1 Ligate (Xba1 and Spe1 Have compatible Overhangs) EcoR1 Xba1 PartB Mixed PartA Spe1 PstI Site A CTAGA TGATC T

Modified BioBrick-Like Strategy AvrII Nhe1 Spe1/Xba1 Mixed Site ACTAGA TGATCT EcoR1 Xba1 Spe1 Pst1 Thr Arg Modified BioBrick EcoR1 Spe1 Nhe1 Pst1 GCT AGT Ala Ser