Fig. 2 Nucleation is maintained but spreading is inhibited by roscovitine treatment. Nucleation is maintained but spreading is inhibited by roscovitine.

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Fig. 2 Nucleation is maintained but spreading is inhibited by roscovitine treatment. Nucleation is maintained but spreading is inhibited by roscovitine treatment. (A) FLC expression after 6 weeks of cold in wild-type (FRI) or a clf mutant, with or without subsequent roscovitine treatment in warm conditions. Data are represented relative to UBC, with each genotype normalized to its respective nonvernalized (NV) FLC level. Error bars represent SEM (n = 4 biological replicates). (B) H3K27me3 levels at FLC after 6 weeks of cold, with or without subsequent roscovitine treatment in warm conditions. Data are expressed relative to STM. Error bars represent SEM (n ≥ 3 biological replicates). Dark shades (black and purple) represent roscovitine treatment, whereas light shades (gray and light purple) represent untreated samples. Curves are fitted by using LOESS regression (supplementary materials). Hongchun Yang et al. Science 2017;357:1142-1145 Copyright © 2017, American Association for the Advancement of Science