MicroRNA-22-3p is down-regulated in the plasma of Han Chinese patients with premature ovarian failure  Yujie Dang, M.D., Ph.D., Shidou Zhao, Ph.D., Yingying Qin, M.D., Ph.D., Ting Han, M.D., Ph.D., Weiping Li, M.D., Ph.D., Zi-Jiang Chen, M.D., Ph.D.  Fertility and Sterility  Volume 103, Issue 3, Pages 802-807.e1 (March 2015) DOI: 10.1016/j.fertnstert.2014.12.106 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Flow diagram of experimental design. BMI = body mass index; POF = premature ovarian failure. Fertility and Sterility 2015 103, 802-807.e1DOI: (10.1016/j.fertnstert.2014.12.106) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Validation of miRNA expression by means of quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in plasma samples from patients with premature ovarian failure (POF; n = 50; red) and control subjects (n = 50; blue). (B) Validation of the expression of miR-22 by means of qRT-PCR on an independent group of plasma samples from POF patients (n = 80; red) and control subjects (n = 80; blue). The expression level of miRNA is presented as fold change relative to the women without POF (control plasma = 1). Data are depicted by scatter plots, and the lines represent the median values. The P values were analyzed by means of the Mann-Whitney U test. (C) Receiver operating characteristic (ROC) analysis of miR-22 for discriminating POF: Plasma miR-22 yields an area under the ROC curve of 0.668 (95% confidence interval 0.602–0.733). Fertility and Sterility 2015 103, 802-807.e1DOI: (10.1016/j.fertnstert.2014.12.106) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 The Gene Ontology analysis of predicted miRNA gene targets. Fertility and Sterility 2015 103, 802-807.e1DOI: (10.1016/j.fertnstert.2014.12.106) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions